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Sequencing ultra-long DNA molecules with the Oxford Nanopore MinION

John M. Urban, Jacob Bliss, Charles E. Lawrence, Susan A. Gerbi
doi: https://doi.org/10.1101/019281
John M. Urban
1BioMed Division (MCB Department), Brown University, Providence, RI, USA
3Center for Computational Molecular Biology, Brown University, Providence, RI, USA
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  • For correspondence: John_Urban@Brown.edu Susan_Gerbi@Brown.edu
Jacob Bliss
1BioMed Division (MCB Department), Brown University, Providence, RI, USA
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Charles E. Lawrence
2Division of Applied Mathematics, Brown University, Providence, RI, USA
3Center for Computational Molecular Biology, Brown University, Providence, RI, USA
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Susan A. Gerbi
1BioMed Division (MCB Department), Brown University, Providence, RI, USA
3Center for Computational Molecular Biology, Brown University, Providence, RI, USA
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  • For correspondence: John_Urban@Brown.edu Susan_Gerbi@Brown.edu
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ABSTRACT

Oxford Nanopore Technologies’ nanopore sequencing device, the MinION, holds the promise of sequencing ultra-long DNA fragments >100kb. An obstacle to realizing this promise is delivering ultra-long DNA molecules to the nanopores. We present our progress in developing cost-effective ways to overcome this obstacle and our resulting MinION data, including multiple reads >100kb.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted May 20, 2015.
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Sequencing ultra-long DNA molecules with the Oxford Nanopore MinION
John M. Urban, Jacob Bliss, Charles E. Lawrence, Susan A. Gerbi
bioRxiv 019281; doi: https://doi.org/10.1101/019281
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Sequencing ultra-long DNA molecules with the Oxford Nanopore MinION
John M. Urban, Jacob Bliss, Charles E. Lawrence, Susan A. Gerbi
bioRxiv 019281; doi: https://doi.org/10.1101/019281

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