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The Cf-4 receptor-like protein associates with the BAK1 receptor-like kinase to initiate receptor endocytosis and plant immunity

Jelle Postma, Thomas W. H. Liebrand, Guozhi Bi, Alexandre Evrard, Ruby R. Bye, Malick Mbengue, Matthieu H. A. J. Joosten, Silke Robatzek
doi: https://doi.org/10.1101/019471
Jelle Postma
1The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
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Thomas W. H. Liebrand
2Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
3Present address: Department of Plant Pathology, 576 Hutchison Hall, University of California, Davis, USA
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Guozhi Bi
2Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
4College of Horticulture, Northeast Agricultural University, Harbin 150030, China
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Alexandre Evrard
1The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
5Present address: Vilmorin SA. Route du Manoir, 49250 La Ménitré, France
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Ruby R. Bye
2Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
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Malick Mbengue
1The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
6Present address: French National Institute for Agricultural Research, Centre de Recherche de Toulouse, France
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Matthieu H. A. J. Joosten
2Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
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  • For correspondence: Matthieu.Joosten@wur.nl Robatzek@TSL.ac.uk
Silke Robatzek
1The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
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  • For correspondence: Matthieu.Joosten@wur.nl Robatzek@TSL.ac.uk
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Summary

  • The first layer of plant immunity is activated by cell surface receptor-like kinases (RLKs) and proteins (RLPs) that detect infectious pathogens. Constitutive interaction with the RLK SUPPRESSOR OF BIR1 (SOBIR1) contributes to RLP stability and kinase activity. As RLK activation requires transphosphorylation with a second associated RLK, it remains elusive how RLPs initiate downstream signaling. To address this, we investigated functioning of Cf RLPs that mediate immunity of tomato against Cladosporium fulvum.

  • We employed live-cell imaging and co-immunoprecipitation in tomato and Nicotiana benthamiana to investigate the requirement of associated kinases for Cf activity and ligand-induced subcellular trafficking of Cf-4.

  • Upon elicitation with the matching effector ligands Avr4 and Avr9, BRI1-ASSOCIATED KINASE 1 (BAK1) associates with Cf-4 and Cf-9. Furthermore, Cf-4 that interacts with SOBIR1 at the plasma membrane, is recruited to late endosomes after elicitation. Significantly, BAK1 is required for Avr4-triggered endocytosis, effector-triggered defenses in Cf-4 plants and resistance of tomato against C. fulvum.

  • Our observations indicate that RLP-mediated immune signaling and endocytosis require ligand-induced recruitment of BAK1, reminiscent of BAK1 interaction and subcellular fate of the FLAGELLIN SENSING 2 RLK. This reveals that diverse classes of cell surface immune receptors share common requirements for signaling initiation and endocytosis.

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Posted May 20, 2015.
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The Cf-4 receptor-like protein associates with the BAK1 receptor-like kinase to initiate receptor endocytosis and plant immunity
Jelle Postma, Thomas W. H. Liebrand, Guozhi Bi, Alexandre Evrard, Ruby R. Bye, Malick Mbengue, Matthieu H. A. J. Joosten, Silke Robatzek
bioRxiv 019471; doi: https://doi.org/10.1101/019471
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The Cf-4 receptor-like protein associates with the BAK1 receptor-like kinase to initiate receptor endocytosis and plant immunity
Jelle Postma, Thomas W. H. Liebrand, Guozhi Bi, Alexandre Evrard, Ruby R. Bye, Malick Mbengue, Matthieu H. A. J. Joosten, Silke Robatzek
bioRxiv 019471; doi: https://doi.org/10.1101/019471

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