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Rust fungal effectors mimic host transit peptides to translocate into chloroplasts

Benjamin Petre, Cécile Lorrain, Diane G.O. Saunders, Sébastien Duplessis, Sophien Kamoun
doi: https://doi.org/10.1101/019521
Benjamin Petre
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
2INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorrain, 54280 Champenoux, France
3Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France
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Cécile Lorrain
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
2INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorrain, 54280 Champenoux, France
3Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France
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Diane G.O. Saunders
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
4The Genome Analysis Centre, Norwich Research Park, NR4 7UH Norwich, United Kingdom
5The John Innes Centre, Norwich Research Park, NR4 7UH Norwich, United Kingdom
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Sébastien Duplessis
2INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorrain, 54280 Champenoux, France
3Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France
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Sophien Kamoun
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
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SUMMARY

Parasite effector proteins target various host cell compartments to alter host processes and promote infection. How effectors cross membrane-rich interfaces to reach these compartments is a major question in effector biology. Growing evidence suggests that effectors use molecular mimicry to subvert host cell machinery for protein sorting. We recently identified CTP1 (chloroplast-targeted protein 1), a candidate effector from the poplar leaf rust fungus Melampsora larici-populina that carries a predicted transit peptide and accumulates in chloroplasts. Here, we show that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts, and is cleaved after translocation. CTP1 is part of a Melampsora-specific family of polymorphic secreted proteins whose members translocate and are processed in chloroplasts in a N-terminal signal-dependent manner. Our findings reveal that fungi have evolved effector proteins that mimic plant-specific sorting signals to traffic within plant cells.

Footnotes

  • ↵* Sophien Kamoun, The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom sophien.kamoun{at}tsl.ac.uk

  • ↵* Sébastien Duplessis, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorrain, 54280 Champenoux, France duplessi{at}nancy.inra.fr

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 23, 2015.
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Rust fungal effectors mimic host transit peptides to translocate into chloroplasts
Benjamin Petre, Cécile Lorrain, Diane G.O. Saunders, Sébastien Duplessis, Sophien Kamoun
bioRxiv 019521; doi: https://doi.org/10.1101/019521
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Rust fungal effectors mimic host transit peptides to translocate into chloroplasts
Benjamin Petre, Cécile Lorrain, Diane G.O. Saunders, Sébastien Duplessis, Sophien Kamoun
bioRxiv 019521; doi: https://doi.org/10.1101/019521

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