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Determining Exon Connectivity in Complex mRNAs by Nanopore Sequencing

Mohan T. Bolisetty, Gopinath Rajadinakaran, Brenton R. Graveley
doi: https://doi.org/10.1101/019752
Mohan T. Bolisetty
1Department of Genetics and Genome Sciences, Institute for Systems Genomics, University of Connecticut Health Center, Farmington, CT 06030
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Gopinath Rajadinakaran
1Department of Genetics and Genome Sciences, Institute for Systems Genomics, University of Connecticut Health Center, Farmington, CT 06030
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Brenton R. Graveley
1Department of Genetics and Genome Sciences, Institute for Systems Genomics, University of Connecticut Health Center, Farmington, CT 06030
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  • For correspondence: graveley@uchc.edu
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Abstract

Though powerful, short-read high throughput RNA sequencing is limited in its ability to directly measure exon connectivity in mRNAs containing multiple alternative exons located farther apart than the maximum read lengths. Here, we use the Oxford Nanopore MinION™ sequencer to identify 7,899 ‘full-length’ isoforms expressed from four Drosophila genes, Dscam1, MRP, Mhc, and Rdl. These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be an important method for comprehensive transcriptome characterization.

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Posted May 22, 2015.
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Determining Exon Connectivity in Complex mRNAs by Nanopore Sequencing
Mohan T. Bolisetty, Gopinath Rajadinakaran, Brenton R. Graveley
bioRxiv 019752; doi: https://doi.org/10.1101/019752
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Determining Exon Connectivity in Complex mRNAs by Nanopore Sequencing
Mohan T. Bolisetty, Gopinath Rajadinakaran, Brenton R. Graveley
bioRxiv 019752; doi: https://doi.org/10.1101/019752

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