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Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum

Artemis Giannakopoulou, John F. C. Steele, Maria Eugenia Segretin, Tolga O. Bozkurt, Ji Zhou, Silke Robatzek, Mark J. Banfield, Marina Pais, Sophien Kamoun
doi: https://doi.org/10.1101/022079
Artemis Giannakopoulou
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
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John F. C. Steele
2Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
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Maria Eugenia Segretin
3INGEBI-CONICET, Ciudad Autónoma de Buenos Aires, C1428ADN, Argentina
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Tolga O. Bozkurt
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
4Imperial College, Faculty of Natural Sciences, Department of Life Sciences, South Kensington Campus, London SW7 2AZ, United Kingdom
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Ji Zhou
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
5The Genome Analysis Centre, Norwich Research Park, NR4 7UH Norwich, United Kingdom
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Silke Robatzek
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
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Mark J. Banfield
2Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
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Marina Pais
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
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  • For correspondence: sophien.kamoun@sainsbury-laboratory.ac.uk marina.pais@sainsbury-laboratory.ac.uk
Sophien Kamoun
1The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
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  • For correspondence: sophien.kamoun@sainsbury-laboratory.ac.uk marina.pais@sainsbury-laboratory.ac.uk
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Abstract

Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat containing proteins (NB-LRR or NLR), to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3aEM, a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the wilt causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2I141N, appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2I141N conferred partial resistance to P. infestans. Further, I2I141N has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared to the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLRs from other plant species.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 07, 2015.
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Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
Artemis Giannakopoulou, John F. C. Steele, Maria Eugenia Segretin, Tolga O. Bozkurt, Ji Zhou, Silke Robatzek, Mark J. Banfield, Marina Pais, Sophien Kamoun
bioRxiv 022079; doi: https://doi.org/10.1101/022079
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Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
Artemis Giannakopoulou, John F. C. Steele, Maria Eugenia Segretin, Tolga O. Bozkurt, Ji Zhou, Silke Robatzek, Mark J. Banfield, Marina Pais, Sophien Kamoun
bioRxiv 022079; doi: https://doi.org/10.1101/022079

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