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Transcriptomic Characterization of Soybean Roots in Response to Bradyrhizobium Infection by RNA Sequencing

Qingyuan He, Yang Hongyan, Ping Wu, Zhengpeng Li, Songhua Wang, Changwei Zhu
doi: https://doi.org/10.1101/024224
Qingyuan He
1College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, Anhui, China
2Soybean Research Institute/ National Center for Soybean Improvement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean/ National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
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Yang Hongyan
2Soybean Research Institute/ National Center for Soybean Improvement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean/ National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
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Ping Wu
1College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, Anhui, China
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Zhengpeng Li
1College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, Anhui, China
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Songhua Wang
1College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, Anhui, China
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Changwei Zhu
1College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, Anhui, China
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ABSTRACT

Legumes interact with rhizobium convert N2 into ammonia for plant use. To investigate the plant basal nitrogen fixation mechanisms induced in response to Bradyrhizobium, differential gene expression in root of inoculated and mock-inoculated soybean was analysed by RNA-Seq. A total of 55787 transcripts were aligned to soybean genome reference sequences, 280 and 316 transcripts were found to be up-and down-regulated, respectively, in inoculated relative to mock-inoculated soybean’s root at V1 stage. Gene ontology (GO) analyses detected 104, 182 and 178 genes associated with cell component category, molecular function category and biological process category, respectively. Pathway analysis revealed that 98 differentiallly expressed genes (115 transcripts) involved in 169 biological pathways. We selected 19 differentially expressed genes and analyzed their expressions in mock-inoculated, inoculated USDA110 and CCBAU45436 using qRT-PCR. The results were consistent with those obtained from rhizobia infected RNA-Seq data. These showed that the results of RNA-Seq have reliability and universality. Additionally, this study showed some novel genes associated with nitrogen fixation process comparison with the previously identified QTLs.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 20, 2015.
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Transcriptomic Characterization of Soybean Roots in Response to Bradyrhizobium Infection by RNA Sequencing
Qingyuan He, Yang Hongyan, Ping Wu, Zhengpeng Li, Songhua Wang, Changwei Zhu
bioRxiv 024224; doi: https://doi.org/10.1101/024224
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Transcriptomic Characterization of Soybean Roots in Response to Bradyrhizobium Infection by RNA Sequencing
Qingyuan He, Yang Hongyan, Ping Wu, Zhengpeng Li, Songhua Wang, Changwei Zhu
bioRxiv 024224; doi: https://doi.org/10.1101/024224

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