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Fasta-O-Matic: a tool to sanity check and if needed reformat FASTA files

View ORCID ProfileJennifer M Shelton, View ORCID ProfileSusan J Brown
doi: https://doi.org/10.1101/024448
Jennifer M Shelton
1KSU/K-INBRE Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, USA
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  • ORCID record for Jennifer M Shelton
Susan J Brown
1KSU/K-INBRE Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, USA
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  • For correspondence: sjbrown@ksu.edu
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Abstract

Background As the sheer volume of bioinformatic sequence data increases, the only way to take advantage of this content is to more completely automate robust analysis workflows. Analysis bottlenecks are often mundane and overlooked processing steps. Idiosyncrasies in reading and/or writing bioinformatics file formats can halt or impair analysis workflows by interfering with the transfer of data from one informatics tools to another.

Results Fasta-O-Matic automates handling of common but minor format issues that otherwise may halt pipelines. The need for automation must be balanced by the need for manual confirmation that any formatting error is actually minor rather than indicative of a corrupt data file. To that end Fasta-O-Matic reports any issues detected to the user with optionally color coded and quiet or verbose logs.

Fasta-O-Matic can be used as a general pre-processing tool in bioinformatics workflows (e.g. to automatically wrap FASTA files so that they can be read by BioPerl). It was also developed as a sanity check for bioinformatic core facilities that tend to repeat common analysis steps on FASTA files received from disparate sources. Fasta-O-Matic can be set with format requirements specific to downstream tools as a first step in a larger analysis workflow.

Availability Fasta-O-Matic is available free of charge to academic and non-profit institutions on GitHub.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted August 21, 2015.
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Fasta-O-Matic: a tool to sanity check and if needed reformat FASTA files
Jennifer M Shelton, Susan J Brown
bioRxiv 024448; doi: https://doi.org/10.1101/024448
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Fasta-O-Matic: a tool to sanity check and if needed reformat FASTA files
Jennifer M Shelton, Susan J Brown
bioRxiv 024448; doi: https://doi.org/10.1101/024448

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