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Growing neuronal islands on multi-electrode arrays using an Accurate Positioning-µCP device

Robert Samhaber, Manuel Schottdorf, Ahmed El Hady, Kai Bröking, Andreas Daus, Christiane Thielemann, Walter Stühmer, Fred Wolf
doi: https://doi.org/10.1101/026401
Robert Samhaber
aMax-Planck-Institute of Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Gottingen, Germany
bMax Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany
dBernstein Center for Computational Neuroscience, Göttingen, Germany
eBernstein Focus Neurotechnology, Göttingen, Germany
fSFB-889 Cellular Mechanisms of Sensory Processing, Göttingen, Germany
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Manuel Schottdorf
aMax-Planck-Institute of Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Gottingen, Germany
bMax Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany
dBernstein Center for Computational Neuroscience, Göttingen, Germany
eBernstein Focus Neurotechnology, Göttingen, Germany
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  • For correspondence: manuel@nld.ds.mpg.de fred@nld.ds.mpg.de
Ahmed El Hady
aMax-Planck-Institute of Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Gottingen, Germany
bMax Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany
dBernstein Center for Computational Neuroscience, Göttingen, Germany
eBernstein Focus Neurotechnology, Göttingen, Germany
fSFB-889 Cellular Mechanisms of Sensory Processing, Göttingen, Germany
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Kai Bröking
aMax-Planck-Institute of Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Gottingen, Germany
bMax Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany
dBernstein Center for Computational Neuroscience, Göttingen, Germany
eBernstein Focus Neurotechnology, Göttingen, Germany
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Andreas Daus
cFaculty of Engineering, University of Applied Science, Würzburger Straße 45, 63743 Aschaffenburg, Germany
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Christiane Thielemann
cFaculty of Engineering, University of Applied Science, Würzburger Straße 45, 63743 Aschaffenburg, Germany
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Walter Stühmer
aMax-Planck-Institute of Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Gottingen, Germany
eBernstein Focus Neurotechnology, Göttingen, Germany
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Fred Wolf
bMax Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany
dBernstein Center for Computational Neuroscience, Göttingen, Germany
eBernstein Focus Neurotechnology, Göttingen, Germany
fSFB-889 Cellular Mechanisms of Sensory Processing, Göttingen, Germany
gFaculty of Physics, Georg-August-Universität Göttingen, Germany
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  • For correspondence: manuel@nld.ds.mpg.de fred@nld.ds.mpg.de
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ABSTRACT

Background Multi-electrode arrays (MEAs) allow non-invasive multiunit recording in-vitro from cultured neuronal networks. For sufficient neuronal growth and adhesion on such MEAs, substrate preparation is required. Plating of dissociated neurons on a uniformly prepared MEA’s surface results in the formation of spatially extended random networks with substantial inter-sample variability. Such cultures are not optimally suited to study the relationship between defined structure and dynamics in neuronal networks. To overcome these shortcomings, neurons can be cultured with pre-defined topology by spatially structured surface modification. Spatially structuring a MEA surface accurately and reproducibly with the equipment of a typical cell-culture laboratory is challenging.

New Method In this paper, we present a novel approach utilizing microcontact printing (μCP) combined with a custom-made device to accurately position patterns on MEAs with high precision. We call this technique AP-μCP (accurate positioning micro-contact printing).

Comparison with existing Methods Other approaches presented in the literature using μCP for patterning either relied on facilities or techniques not readily available in a standard cell culture laboratory, or they did not specify means of precise pattern positioning.

Conclusion Here we present a relatively simple device for reproducible and precise patterning in a standard cell-culture laboratory setting. The patterned neuronal islands on MEAs provide a basis for high throughput electrophysiology to study the dynamics of single neurons and neuronal networks.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 01, 2015.
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Growing neuronal islands on multi-electrode arrays using an Accurate Positioning-µCP device
Robert Samhaber, Manuel Schottdorf, Ahmed El Hady, Kai Bröking, Andreas Daus, Christiane Thielemann, Walter Stühmer, Fred Wolf
bioRxiv 026401; doi: https://doi.org/10.1101/026401
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Growing neuronal islands on multi-electrode arrays using an Accurate Positioning-µCP device
Robert Samhaber, Manuel Schottdorf, Ahmed El Hady, Kai Bröking, Andreas Daus, Christiane Thielemann, Walter Stühmer, Fred Wolf
bioRxiv 026401; doi: https://doi.org/10.1101/026401

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