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Divergent MLS1 promoters lie on a fitness plateau for gene expression

Andrew C. Bergen, Gerilyn M. Olsen, Justin C. Fay
doi: https://doi.org/10.1101/026427
Andrew C. Bergen
1Molecular Genetics and Genomics Program, Washington University, St. Louis, MO
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Gerilyn M. Olsen
2Washington University, St. Louis, MO
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Justin C. Fay
3Department of Genetics, Washington University, St. Louis, MO
4Center for Genome Sciences and Systems Biology, Washington University, St. Louis, MO
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ABSTRACT

Qualitative patterns of gene activation and repression are often conserved despite an abundance of quantitative variation in expression levels within and between species. A major challenge to interpreting patterns of expression divergence is knowing which changes in gene expression affect fitness. To characterize the fitness effects of gene expression divergence we placed orthologous promoters from eight yeast species upstream of malate synthase (MLS1) in Saccharomyces cerevisiae. As expected, we found these promoters varied in their expression level under activated and repressed conditions as well as in their dynamic response following loss of glucose repression. Despite these differences, only a single promoter driving near basal levels of expression caused a detectable loss of fitness. We conclude that the MLS1 promoter lies on a fitness plateau whereby even large changes in gene expression can be tolerated without a substantial loss of fitness.

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Posted September 09, 2015.
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Divergent MLS1 promoters lie on a fitness plateau for gene expression
Andrew C. Bergen, Gerilyn M. Olsen, Justin C. Fay
bioRxiv 026427; doi: https://doi.org/10.1101/026427
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Divergent MLS1 promoters lie on a fitness plateau for gene expression
Andrew C. Bergen, Gerilyn M. Olsen, Justin C. Fay
bioRxiv 026427; doi: https://doi.org/10.1101/026427

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