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Complete assembly of novel environmental bacterial genomes by MinIONTM sequencing

Daniel J Turner, Xiaoguang Dai, Simon C Mayes, Sissel Juul
doi: https://doi.org/10.1101/026930
Daniel J Turner
1Oxford Nanopore Technologies Ltd., Oxford, UK
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  • For correspondence: daniel.turner@nanoporetech.com
Xiaoguang Dai
2Oxford Nanopore Technologies Inc., New York, USA
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Simon C Mayes
1Oxford Nanopore Technologies Ltd., Oxford, UK
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Sissel Juul
2Oxford Nanopore Technologies Inc., New York, USA
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Abstract

In this study, we adapt a protocol for the growth of previously uncultured environmental bacterial isolates, to make it compatible with whole genome sequencing. We demonstrate that in combination with the MinION sequencing device, complete assemblies can be derived, allowing genomic comparisons to be made. This approach allows rapid, inexpensive and straightforward discovery, and genomic analysis, of previously uncultured prokaryotic genomes, and brings greater ownership of all parts of the sequencing process back to individual researchers.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 19, 2015.
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Complete assembly of novel environmental bacterial genomes by MinIONTM sequencing
Daniel J Turner, Xiaoguang Dai, Simon C Mayes, Sissel Juul
bioRxiv 026930; doi: https://doi.org/10.1101/026930
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Complete assembly of novel environmental bacterial genomes by MinIONTM sequencing
Daniel J Turner, Xiaoguang Dai, Simon C Mayes, Sissel Juul
bioRxiv 026930; doi: https://doi.org/10.1101/026930

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