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Cannabis microbiome sequencing reveals several mycotoxic fungi native to dispensary grade cannabis flowers

View ORCID ProfileKevin McKernan, View ORCID ProfileJessica Spangler, View ORCID ProfileLei Zhang, View ORCID ProfileVasisht Tadigotla, View ORCID ProfileYvonne Helbert, View ORCID ProfileTheodore Foss, View ORCID ProfileDouglas Smith
doi: https://doi.org/10.1101/030775
Kevin McKernan
Medicinal Genomics Corporation, Woburn MA, 01801, USA
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Jessica Spangler
Medicinal Genomics Corporation, Woburn MA, 01801, USA
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Lei Zhang
Medicinal Genomics Corporation, Woburn MA, 01801, USA
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Vasisht Tadigotla
Medicinal Genomics Corporation, Woburn MA, 01801, USA
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Yvonne Helbert
Medicinal Genomics Corporation, Woburn MA, 01801, USA
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Theodore Foss
Medicinal Genomics Corporation, Woburn MA, 01801, USA
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Douglas Smith
Medicinal Genomics Corporation, Woburn MA, 01801, USA
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Abstract

The Center for Disease Control estimates 128,000 people in the U.S. are hospitalized annually due to food borne illnesses. This has created a demand for food safety testing targeting the detection of pathogenic mold and bacteria on agricultural products. This risk extends to medical Cannabis and is of particular concern with inhaled, vaporized and even concentrated Cannabis products. As a result, third party microbial testing has become a regulatory requirement in the medical and recreational Cannabis markets, yet knowledge of the Cannabis microbiome is limited. Here we describe the first next generation sequencing survey of the microbial communities found in dispensary based Cannabis flowers and demonstrate the limitations in the culture based regulations that are being superimposed from the food industry.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted November 06, 2015.
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Cannabis microbiome sequencing reveals several mycotoxic fungi native to dispensary grade cannabis flowers
Kevin McKernan, Jessica Spangler, Lei Zhang, Vasisht Tadigotla, Yvonne Helbert, Theodore Foss, Douglas Smith
bioRxiv 030775; doi: https://doi.org/10.1101/030775
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Cannabis microbiome sequencing reveals several mycotoxic fungi native to dispensary grade cannabis flowers
Kevin McKernan, Jessica Spangler, Lei Zhang, Vasisht Tadigotla, Yvonne Helbert, Theodore Foss, Douglas Smith
bioRxiv 030775; doi: https://doi.org/10.1101/030775

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