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FecalSeq: methylation-based enrichment for noninvasive population genomics from feces

View ORCID ProfileKenneth L. Chiou, View ORCID ProfileChristina M. Bergey
doi: https://doi.org/10.1101/032870
Kenneth L. Chiou
1Department of Anthropology, Washington University, St. Louis, MO 63130, USA
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Christina M. Bergey
2Department of Anthropology, New York University, New York, NY 10003, USA
3New York Consortium in Evolutionary Primatology, New York, NY, USA
4Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
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Abstract

We developed an inexpensive methylation-based capture method for enriching host DNA from noninvasively obtained fecal samples. We demonstrate that the enrichment is robust, efficient, and compatible with downstream library preparation methods, including complexity-reduction approaches for massively parallel sequencing. Because feces are widely available and convenient to collect, our method empowers researchers to explore genomic-scale population-level questions in organisms for which invasive sampling is challenging or undesirable.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 25, 2015.
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FecalSeq: methylation-based enrichment for noninvasive population genomics from feces
Kenneth L. Chiou, Christina M. Bergey
bioRxiv 032870; doi: https://doi.org/10.1101/032870
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FecalSeq: methylation-based enrichment for noninvasive population genomics from feces
Kenneth L. Chiou, Christina M. Bergey
bioRxiv 032870; doi: https://doi.org/10.1101/032870

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