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The Arabidopsis Auxin Receptor F-box proteins AFB4 and AFB5 are Required for Response to the Synthetic Auxin Picloram

Michael J. Prigge, Kathleen Greenham, Yi Zhang, Aaron Santner, Cristina Castillejo, Ronan C. O’Malley, Joseph R. Ecker, Mark Estelle
doi: https://doi.org/10.1101/034652
Michael J. Prigge
1Section of Cell and Developmental Biology and Howard Hughes Medical Institute, University of California San Diego La Jolla CA 92093.
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Kathleen Greenham
1Section of Cell and Developmental Biology and Howard Hughes Medical Institute, University of California San Diego La Jolla CA 92093.
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Yi Zhang
1Section of Cell and Developmental Biology and Howard Hughes Medical Institute, University of California San Diego La Jolla CA 92093.
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Aaron Santner
1Section of Cell and Developmental Biology and Howard Hughes Medical Institute, University of California San Diego La Jolla CA 92093.
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Cristina Castillejo
1Section of Cell and Developmental Biology and Howard Hughes Medical Institute, University of California San Diego La Jolla CA 92093.
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Ronan C. O’Malley
2Genomic Analysis Laboratory, Howard Hughes Medical Institute and The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Joseph R. Ecker
2Genomic Analysis Laboratory, Howard Hughes Medical Institute and The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
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Mark Estelle
1Section of Cell and Developmental Biology and Howard Hughes Medical Institute, University of California San Diego La Jolla CA 92093.
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  • For correspondence: mestelle@ucsd.edu
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Abstract

The plant hormone auxin is perceived by a family of F-box proteins called the TIR1/AFBs. Phylogenetic studies reveal that these proteins fall into four clades in flowering plants called TIR1, AFB2, AFB4, and AFB6 (Parry et al. 2009). Genetic studies indicate that members of the TIR1 and AFB2 groups act as positive regulators of auxin signaling by promoting the degradation of the Aux/IAA transcriptional repressors (Dharmasiri et al. 2005; Parry et al. 2009). In this report, we demonstrate that both AFB4 and AFB5 also function as auxin receptors based on in vitro assays. We also provide genetic evidence that both AFB4 and AFB5 are targets of the picloram family of auxinic herbicides. In contrast to previous studies we find that null afb4 alleles do not exhibit obvious defects in seedling morphology or auxin hypersensitivity. We conclude that AFB4 and AFB5 act in a similar fashion to other members of the family but exhibit a distinct auxin specificity.

Footnotes

  • ↵3 Current addres: Department of Biological Sciences, 78 College Street, Dartmouth College Hanover, NH 03755

  • ↵4 Current addres: Cook Inc., Bloomington, IN 47402, USA

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 18, 2015.
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The Arabidopsis Auxin Receptor F-box proteins AFB4 and AFB5 are Required for Response to the Synthetic Auxin Picloram
Michael J. Prigge, Kathleen Greenham, Yi Zhang, Aaron Santner, Cristina Castillejo, Ronan C. O’Malley, Joseph R. Ecker, Mark Estelle
bioRxiv 034652; doi: https://doi.org/10.1101/034652
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The Arabidopsis Auxin Receptor F-box proteins AFB4 and AFB5 are Required for Response to the Synthetic Auxin Picloram
Michael J. Prigge, Kathleen Greenham, Yi Zhang, Aaron Santner, Cristina Castillejo, Ronan C. O’Malley, Joseph R. Ecker, Mark Estelle
bioRxiv 034652; doi: https://doi.org/10.1101/034652

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