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Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex

Mark A. DeWitt, Wendy Magis, Nicolas L. Bray, Tianjiao Wang, Jennifer R. Berman, Fabrizia Urbinati, Denise P. Muñoz, Donald B. Kohn, Mark C. Walters, Dana Carroll, David K. Martin, Jacob E. Corn
doi: https://doi.org/10.1101/036236
Mark A. DeWitt
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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Wendy Magis
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
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Nicolas L. Bray
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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Tianjiao Wang
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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Jennifer R. Berman
4Digital Biology Center, Bio-Rad Laboratories, Pleasanton, CA, USA.
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Fabrizia Urbinati
5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA
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Denise P. Muñoz
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
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Donald B. Kohn
5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA
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Mark C. Walters
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
6Blood & Marrow Program, Division of Hematology/Oncology/BMT, UCSF Benioff Children’s Hospital, Oakland, Oakland, CA, USA
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Dana Carroll
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
7Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA
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  • For correspondence: jcorn@berkeley.edu dimartin@chori.org dana@biochem.utah.edu
David K. Martin
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
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  • For correspondence: jcorn@berkeley.edu dimartin@chori.org dana@biochem.utah.edu
Jacob E. Corn
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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  • For correspondence: jcorn@berkeley.edu dimartin@chori.org dana@biochem.utah.edu
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Article Information

doi 
https://doi.org/10.1101/036236
History 
  • January 15, 2016.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Mark A. DeWitt1,2,
  2. Wendy Magis3,
  3. Nicolas L. Bray1,2,
  4. Tianjiao Wang1,2,
  5. Jennifer R. Berman4,
  6. Fabrizia Urbinati5,
  7. Denise P. Muñoz3,
  8. Donald B. Kohn5,
  9. Mark C. Walters3,6,
  10. Dana Carroll1,7,*,
  11. David K. Martin3,* and
  12. Jacob E. Corn1,2,*
  1. 1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
  2. 2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
  3. 3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
  4. 4Digital Biology Center, Bio-Rad Laboratories, Pleasanton, CA, USA.
  5. 5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA
  6. 6Blood & Marrow Program, Division of Hematology/Oncology/BMT, UCSF Benioff Children’s Hospital, Oakland, Oakland, CA, USA
  7. 7Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA
  1. ↵* Co-corresponding authors: Jacob Corn jcorn{at}berkeley.edu, David Martin dimartin{at}chori.org and Dana Carroll dana{at}biochem.utah.edu
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Posted January 15, 2016.
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Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex
Mark A. DeWitt, Wendy Magis, Nicolas L. Bray, Tianjiao Wang, Jennifer R. Berman, Fabrizia Urbinati, Denise P. Muñoz, Donald B. Kohn, Mark C. Walters, Dana Carroll, David K. Martin, Jacob E. Corn
bioRxiv 036236; doi: https://doi.org/10.1101/036236
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Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex
Mark A. DeWitt, Wendy Magis, Nicolas L. Bray, Tianjiao Wang, Jennifer R. Berman, Fabrizia Urbinati, Denise P. Muñoz, Donald B. Kohn, Mark C. Walters, Dana Carroll, David K. Martin, Jacob E. Corn
bioRxiv 036236; doi: https://doi.org/10.1101/036236

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