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Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex

Mark A. DeWitt, Wendy Magis, Nicolas L. Bray, Tianjiao Wang, Jennifer R. Berman, Fabrizia Urbinati, Denise P. Muñoz, Donald B. Kohn, Mark C. Walters, Dana Carroll, David K. Martin, Jacob E. Corn
doi: https://doi.org/10.1101/036236
Mark A. DeWitt
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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Wendy Magis
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
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Nicolas L. Bray
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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Tianjiao Wang
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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Jennifer R. Berman
4Digital Biology Center, Bio-Rad Laboratories, Pleasanton, CA, USA.
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Fabrizia Urbinati
5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA
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Denise P. Muñoz
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
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Donald B. Kohn
5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA
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Mark C. Walters
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
6Blood & Marrow Program, Division of Hematology/Oncology/BMT, UCSF Benioff Children’s Hospital, Oakland, Oakland, CA, USA
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Dana Carroll
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
7Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA
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  • For correspondence: jcorn@berkeley.edu dimartin@chori.org dana@biochem.utah.edu
David K. Martin
3Children’s Hospital Oakland Research Institute, UCSF Benioff Children’s Hospital, Oakland, California, USA
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  • For correspondence: jcorn@berkeley.edu dimartin@chori.org dana@biochem.utah.edu
Jacob E. Corn
1Innovative Genomics Initiative, University of California-Berkeley, CA, USA
2Department of Molecular and Cellular Biology, University of California-Berkeley, CA, USA
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  • For correspondence: jcorn@berkeley.edu dimartin@chori.org dana@biochem.utah.edu
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Posted January 15, 2016.
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Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex
Mark A. DeWitt, Wendy Magis, Nicolas L. Bray, Tianjiao Wang, Jennifer R. Berman, Fabrizia Urbinati, Denise P. Muñoz, Donald B. Kohn, Mark C. Walters, Dana Carroll, David K. Martin, Jacob E. Corn
bioRxiv 036236; doi: https://doi.org/10.1101/036236
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Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex
Mark A. DeWitt, Wendy Magis, Nicolas L. Bray, Tianjiao Wang, Jennifer R. Berman, Fabrizia Urbinati, Denise P. Muñoz, Donald B. Kohn, Mark C. Walters, Dana Carroll, David K. Martin, Jacob E. Corn
bioRxiv 036236; doi: https://doi.org/10.1101/036236

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