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Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging

Simon R. Schultz, Caroline S. Copeland, Amanda J. Foust, Peter Quicke, Renaud Schuck
doi: https://doi.org/10.1101/036632
Simon R. Schultz
Center for Neurotechnology and Department of Bioengineering Imperial College London, South Kensington, London SW7 2AZ, UK e-mail:
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  • For correspondence: s.schultz@imperial.ac.uk
Caroline S. Copeland
Center for Neurotechnology and Department of Bioengineering Imperial College London, South Kensington, London SW7 2AZ, UK e-mail:
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  • For correspondence: s.schultz@imperial.ac.uk
Amanda J. Foust
Center for Neurotechnology and Department of Bioengineering Imperial College London, South Kensington, London SW7 2AZ, UK e-mail:
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  • For correspondence: s.schultz@imperial.ac.uk
Peter Quicke
Center for Neurotechnology and Department of Bioengineering Imperial College London, South Kensington, London SW7 2AZ, UK e-mail:
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  • For correspondence: s.schultz@imperial.ac.uk
Renaud Schuck
Center for Neurotechnology and Department of Bioengineering Imperial College London, South Kensington, London SW7 2AZ, UK e-mail:
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  • For correspondence: s.schultz@imperial.ac.uk
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Abstract

Recent years have seen substantial developments in technology for imaging neural circuits, raising the prospect of large scale imaging studies of neural populations involved in information processing, with the potential to lead to step changes in our understanding of brain function and dysfunction. In this article we will review some key recent advances: improved fluorophores for single cell resolution functional neuroimaging using a two photon microscope; improved approaches to the problem of scanning active circuits; and the prospect of scanless microscopes which overcome some of the bandwidth limitations of current imaging techniques. These advances in technology for experimental neuroscience have in themselves led to technical challenges, such as the need for the development of novel signal processing and data analysis tools in order to make the most of the new experimental tools. We review recent work in some active topics, such as region of interest segmentation algorithms capable of demixing overlapping signals, and new highly accurate algorithms for calcium transient detection. These advances motivate the development of new data analysis tools capable of dealing with spatial or spatiotem-poral patterns of neural activity, that scale well with pattern size.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted June 03, 2016.
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Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging
Simon R. Schultz, Caroline S. Copeland, Amanda J. Foust, Peter Quicke, Renaud Schuck
bioRxiv 036632; doi: https://doi.org/10.1101/036632
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Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging
Simon R. Schultz, Caroline S. Copeland, Amanda J. Foust, Peter Quicke, Renaud Schuck
bioRxiv 036632; doi: https://doi.org/10.1101/036632

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