Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors

Yoshinao Katsu, Satomi Kohno, Kaori Oka, Michael E. Baker
doi: https://doi.org/10.1101/036665
Yoshinao Katsu
1Graduate School of Life Science, Hokkaido University, Sapporo, Japan;
2Department of Biological Sciences, Hokkaido University, Sapporo, Japan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: ykatsu@sci.hokudai.ac.jp mbaker@ucsd.edu
Satomi Kohno
3Department of Obstetrics and Gynecology, Medical University of South Carolina, and Marine Biomedicine and Environmental Science Center, Hollings Marine Laboratory, Charleston, SC, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kaori Oka
1Graduate School of Life Science, Hokkaido University, Sapporo, Japan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael E. Baker
4Department of Medicine, University of California, San Diego, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: ykatsu@sci.hokudai.ac.jp mbaker@ucsd.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

We investigated the evolution of the response of human, chicken, alligator and frog glucocorticoid receptors (GRs) to dexamethasone, cortisol, corticosterone, 11-deoxycorticosterone, 11-deoxycortisol and aldosterone. We find significant differences among these vertebrates in the transcriptional activation of their full length GRs by these steroids, indicating that there were changes in the specificity of the GR for steroids during the evolution of terrestrial vertebrates. To begin to study the role of interactions between different domains on the GR in steroid sensitivity and specificity for terrestrial GRs, we investigated transcriptional activation of truncated GRs containing their hinge domain and ligand binding domain (LBD) fused to a GAL4 DNA binding domain (GAL4 DBD). Compared to corresponding full length GRs, transcriptional activation of GAL4 DBD-GR hinge/LBD constructs required higher steroid concentrations and displayed altered steroid specificity, indicating that interactions between the hinge/LBD and other domains are important in glucocorticoid activation of these terrestrial GRs.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted January 14, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors
Yoshinao Katsu, Satomi Kohno, Kaori Oka, Michael E. Baker
bioRxiv 036665; doi: https://doi.org/10.1101/036665
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors
Yoshinao Katsu, Satomi Kohno, Kaori Oka, Michael E. Baker
bioRxiv 036665; doi: https://doi.org/10.1101/036665

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Evolutionary Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4224)
  • Biochemistry (9101)
  • Bioengineering (6749)
  • Bioinformatics (23935)
  • Biophysics (12086)
  • Cancer Biology (9491)
  • Cell Biology (13728)
  • Clinical Trials (138)
  • Developmental Biology (7614)
  • Ecology (11656)
  • Epidemiology (2066)
  • Evolutionary Biology (15476)
  • Genetics (10615)
  • Genomics (14292)
  • Immunology (9456)
  • Microbiology (22773)
  • Molecular Biology (9069)
  • Neuroscience (48840)
  • Paleontology (354)
  • Pathology (1479)
  • Pharmacology and Toxicology (2562)
  • Physiology (3822)
  • Plant Biology (8307)
  • Scientific Communication and Education (1467)
  • Synthetic Biology (2289)
  • Systems Biology (6170)
  • Zoology (1297)