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Sensitive whole mount in situ localization of small RNAs in plants

Mouli Ghosh Dastidar, Magdalena Mosiolek, Michael D. Nodine, Alexis Maizel
doi: https://doi.org/10.1101/037978
Mouli Ghosh Dastidar
1Center for Organismal Studies (COS), University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
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Magdalena Mosiolek
2Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Michael D. Nodine
2Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Alexis Maizel
1Center for Organismal Studies (COS), University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
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  • For correspondence: alexis.maizel@cos.uni-heidelberg.de
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Abstract

Small regulatory RNAs are pivotal regulators of gene expression and play important roles in many plant processes. Although our knowledge of their biogenesis and mode of action has significantly progressed, we comparatively still know little about their biological functions. In particular, knowledge about their spatiotemporal patterns of expression rely on either indirect detection by use of reporter constructs or labor-intensive direct detection by in situ hybridization on sectioned material. None of the current approaches allows for a systematic investigation of small RNAs expression patterns.Here, we present a method for the sensitive in situ detection of micro-and siRNAs in intact plant tissues that utilizes both double-labelled probes and a specific cross linker. We determined the expression patterns of several small RNAs in plant roots and embryos.

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Posted January 26, 2016.
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Sensitive whole mount in situ localization of small RNAs in plants
Mouli Ghosh Dastidar, Magdalena Mosiolek, Michael D. Nodine, Alexis Maizel
bioRxiv 037978; doi: https://doi.org/10.1101/037978
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Sensitive whole mount in situ localization of small RNAs in plants
Mouli Ghosh Dastidar, Magdalena Mosiolek, Michael D. Nodine, Alexis Maizel
bioRxiv 037978; doi: https://doi.org/10.1101/037978

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