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Bioanalyzer chips can be used interchangeably for many analyses of DNA or RNA

Jessica Davies, Tom Denyer, View ORCID ProfileJames Hadfield
doi: https://doi.org/10.1101/039040
Jessica Davies
1 Genomics Core Facility, Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Robinson Way, Cambridge, CB2 ORE, UK
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Tom Denyer
1 Genomics Core Facility, Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Robinson Way, Cambridge, CB2 ORE, UK
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James Hadfield
1 Genomics Core Facility, Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Robinson Way, Cambridge, CB2 ORE, UK
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  • ORCID record for James Hadfield
  • For correspondence: James.Hadfield@cruk.cam.ac.uk
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Abstract

The Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) enables small-scale gel electrophoretic separation of nucleic acids on a microfluidic chip. Shortage of chips and excess reagents is a common issue. This report explored the compatibility of two commonly used Bioanalyzer reagents with three Bioanalyzer chip types. Microfluidic electrophoretic separation of DNA and RNA using DNA High Sensitivity and RNA 6000 Nano reagents, respectively, was successfully performed on multiple chip types, following the assay-specific protocol. For RNA quality and next-generation sequencing library size estimation, the Bioanalyzer chips tested can be used interchangeably. These findings will be valuable for any laboratory using the Agilent Bioanalyzer in a shared facility.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted February 07, 2016.
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Bioanalyzer chips can be used interchangeably for many analyses of DNA or RNA
Jessica Davies, Tom Denyer, James Hadfield
bioRxiv 039040; doi: https://doi.org/10.1101/039040
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Bioanalyzer chips can be used interchangeably for many analyses of DNA or RNA
Jessica Davies, Tom Denyer, James Hadfield
bioRxiv 039040; doi: https://doi.org/10.1101/039040

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