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Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska, Sofie Wendel, Morten H. H. Nørholm, Antoine Danchin
doi: https://doi.org/10.1101/041525
Agnieszka Sekowska
1AMAbiotics SAS, Brain and Spine Institute, Hôpital de la Pitié-Salpêtrière, 47, Boulevard de l’Hôpital, 75013 Paris, France
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Sofie Wendel
2Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle 6, DK-2970 Hørsholm, Denmark
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Morten H. H. Nørholm
2Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle 6, DK-2970 Hørsholm, Denmark
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  • For correspondence: antoine.danchin@normalesup.org morno@biosustain.dtu.dk
Antoine Danchin
1AMAbiotics SAS, Brain and Spine Institute, Hôpital de la Pitié-Salpêtrière, 47, Boulevard de l’Hôpital, 75013 Paris, France
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  • For correspondence: antoine.danchin@normalesup.org morno@biosustain.dtu.dk
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Abstract

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand.

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Posted February 26, 2016.
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Generation of mutation hotspots in ageing bacterial colonies
Agnieszka Sekowska, Sofie Wendel, Morten H. H. Nørholm, Antoine Danchin
bioRxiv 041525; doi: https://doi.org/10.1101/041525
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Generation of mutation hotspots in ageing bacterial colonies
Agnieszka Sekowska, Sofie Wendel, Morten H. H. Nørholm, Antoine Danchin
bioRxiv 041525; doi: https://doi.org/10.1101/041525

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