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The aptabot: an inducibly affinity-switching, minimally invasive in vivo contrast agent

Elleard Felix Webster Heffern, Jason Fuller, Russell W. Hanson
doi: https://doi.org/10.1101/043950
Elleard Felix Webster Heffern
1Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
2Department of Biochemistry, University of California in Los Angeles, Los Angeles, California, USA
3, 1425 Madison Avenue, Box 1498, New York, NY 100296574
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Jason Fuller
3, 1425 Madison Avenue, Box 1498, New York, NY 100296574
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Russell W. Hanson
3, 1425 Madison Avenue, Box 1498, New York, NY 100296574
4Children’s Hospital Informatics Program at the Harvard–Massachusetts Institute of Technology Division of Health Sciences and Technology, Harvard Medical School, Boston, Massachusetts, USA
5Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, 2800 Lyngby, Denmark
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  • For correspondence: russell.hanson@mssm.edu
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Abstract

The ambitions of current neuroscience—understanding neurological disease progression and mapping the connectome—demonstrate a need for safe in vivo tools for creating intricate maps of brain circuitry. Present in vivo contrast agents are often limited by their specificity, uptake, resolvability, and/or clearance.

We describe an aptamer-functionalized sensor for high-resolution imaging that can switch imaging targets by an induced multi-stage aptamer reaction. Included are synthetic methods as well as calculations of sensor efficacy based on known kinetics. Calculations show that 10 distinct targets may be imaged in a living brain at the submicron scale within 42 hours.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 16, 2016.
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The aptabot: an inducibly affinity-switching, minimally invasive in vivo contrast agent
Elleard Felix Webster Heffern, Jason Fuller, Russell W. Hanson
bioRxiv 043950; doi: https://doi.org/10.1101/043950
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The aptabot: an inducibly affinity-switching, minimally invasive in vivo contrast agent
Elleard Felix Webster Heffern, Jason Fuller, Russell W. Hanson
bioRxiv 043950; doi: https://doi.org/10.1101/043950

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