Abstract
Motivation: In 2014, Oxford Nanopore Technologies (ONT) announced a new sequencing platform called MinION. The particular features of MinION reads – longer read lengths and single-molecule sequencing in particular – show potential for genome characterization. As of yet, the pre-commercial technology is exclusively available through early-access, and only a few datasets are publically available for testing. Further, no software exists that simulates MinION platform reads with genuine ONT characteristics.
Results: In this article, we introduce NanoSim, a fast and scalable read simulator that captures the technology-specific features of ONT data, and allows for adjustments upon improvement of nanopore sequencing technology.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Posted March 18, 2016.
NanoSim: nanopore sequence read simulator based on statistical characterization
