Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Bone marrow mesenchymal stem cells from osteoporotic patients do not show altered mitochondrial energetics or ultrastructure

Clemens W.I. Moll, Richard A. Lindtner, Georg F. Vogel, Martina Brunner, Karin Gutleben, Michael Blauth, Gerhard Krumschnabel, Hannes L. Ebner
doi: https://doi.org/10.1101/046300
Clemens W.I. Moll
1Department for Trauma Surgery, Medical University of Innsbruck, Anichstraβe 35, 6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Richard A. Lindtner
1Department for Trauma Surgery, Medical University of Innsbruck, Anichstraβe 35, 6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Georg F. Vogel
2Division of Histology and Embryology, Medical University of Innsbruck, Müllerstraβe 59, 6020 Innsbruck, Austria
3Division of Cell Biology, Biocenter, Innsbruck Medical University, Innrain 80, 6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Martina Brunner
1Department for Trauma Surgery, Medical University of Innsbruck, Anichstraβe 35, 6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karin Gutleben
2Division of Histology and Embryology, Medical University of Innsbruck, Müllerstraβe 59, 6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Blauth
1Department for Trauma Surgery, Medical University of Innsbruck, Anichstraβe 35, 6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gerhard Krumschnabel
4OROBOROS INSTRUMENTS, Schöpfstraße 18, 6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hannes L. Ebner
1Department for Trauma Surgery, Medical University of Innsbruck, Anichstraβe 35, 6020 Innsbruck, Austria
5Bone and Stem Cell Research Group, CABMM, University of Zurich, Winterthurerstraβe 190, 8057 Zurich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Hannes.ebner@medel.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

As a consequence of the ongoing demographic change, osteoporosis is considered as one of the mayor challenges for the health care system of the 21st century. However, the exact etiology of osteoporosis is far from being understood. Some evidence suggests that changes in stem cell metabolism might contribute to development of the disease. Therefore we evaluated whether differences of the morphology and/or the energy metabolism of mitochondria can be observed between human bone marrow derived mesenchymal stem cells obtained from osteoporotic patients as compared to non-osteoporotic controls.

Mesenchymal stem cells were isolated from the bone marrow of senile osteoporotic and non osteoporotic patients, osteoporosis being assessed by dual energy X-ray absorptiometry. We then confirmed the stemness of the cells by FACS analysis of the expression of surface markers and by conducting multi-lineage differentiation experiments. And we finally investigated mitochondrial morphology and function with electron microscopy of cryo-fixed samples and by high-resolution respirometry, respectively. In addition we compared the energy metabolism of the stem cells to those of the osteosarcoma cell line MG-63.

The data show, for the first time, the applicability on stem cells of the methods used here. Furthermore, our results indicated that there are no obvious differences detectable in mitochondrial morphology between cells from osteoporotic and non osteoporotic donors and that these cells also seem to be energetically indistinguishable with unchanged rates of routine respiration and respiratory capacity as well as unaltered oxygen consumption rates linked to different respiratory complexes. In summary, we could not detect any evidence indicating major changes of mitochondrial features in cells from osteoporotic patients.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 30, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Bone marrow mesenchymal stem cells from osteoporotic patients do not show altered mitochondrial energetics or ultrastructure
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Bone marrow mesenchymal stem cells from osteoporotic patients do not show altered mitochondrial energetics or ultrastructure
Clemens W.I. Moll, Richard A. Lindtner, Georg F. Vogel, Martina Brunner, Karin Gutleben, Michael Blauth, Gerhard Krumschnabel, Hannes L. Ebner
bioRxiv 046300; doi: https://doi.org/10.1101/046300
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Bone marrow mesenchymal stem cells from osteoporotic patients do not show altered mitochondrial energetics or ultrastructure
Clemens W.I. Moll, Richard A. Lindtner, Georg F. Vogel, Martina Brunner, Karin Gutleben, Michael Blauth, Gerhard Krumschnabel, Hannes L. Ebner
bioRxiv 046300; doi: https://doi.org/10.1101/046300

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3586)
  • Biochemistry (7545)
  • Bioengineering (5495)
  • Bioinformatics (20732)
  • Biophysics (10294)
  • Cancer Biology (7951)
  • Cell Biology (11611)
  • Clinical Trials (138)
  • Developmental Biology (6586)
  • Ecology (10168)
  • Epidemiology (2065)
  • Evolutionary Biology (13580)
  • Genetics (9521)
  • Genomics (12817)
  • Immunology (7906)
  • Microbiology (19503)
  • Molecular Biology (7641)
  • Neuroscience (41982)
  • Paleontology (307)
  • Pathology (1254)
  • Pharmacology and Toxicology (2192)
  • Physiology (3259)
  • Plant Biology (7025)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1947)
  • Systems Biology (5419)
  • Zoology (1113)