ABSTRACT
Failure to detect low-abundance microRNAs (miRNAs), for example, in circulating plasma, may occur for a variety of reasons, including presence of enzyme inhibitors. Recently, we received the unusual but intriguing suggestion that miR-16-5p acts as a co-factor of reverse transcriptases, facilitating more efficient reverse transcription of miRNAs and thus enhanced detection of low-abundance miRNAs. We tested this hypothesis by incubating reverse transcriptase with several concentrations of synthetic miR-16-5p and then performing stem-loop RT-qPCR with serial dilutions of miRNA osa-MIR168a. Our results do not support a role for miR-16 as a co-factor of reverse transcriptase.
Copyright
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Posted April 04, 2016.
Synthetic miR-16-5p does not act as a reverse transcriptase co-factor to enhance detection of small RNA
