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Biphasic growth dynamics during Caulobacter crescentus division

Shiladitya Banerjee, Klevin Lo, Matthew K. Daddysman, Alan Selewa, Thomas Kuntz, Aaron R. Dinner, Norbert F. Scherer
doi: https://doi.org/10.1101/047589
Shiladitya Banerjee
1 James Franck Institute, The University of Chicago, Chicago IL 60637, USA
2 Department of Physics and Astronomy, University College London, London WC1E 6BT, UK
3 Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UK
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Klevin Lo
1 James Franck Institute, The University of Chicago, Chicago IL 60637, USA
4 Institute for Biophysical Dynamics, The University of Chicago, Chicago IL 60637, USA
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Matthew K. Daddysman
4 Institute for Biophysical Dynamics, The University of Chicago, Chicago IL 60637, USA
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Alan Selewa
4 Institute for Biophysical Dynamics, The University of Chicago, Chicago IL 60637, USA
5 Biophysical Sciences Graduate Program, The University of Chicago, Chicago IL 60637, USA
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Thomas Kuntz
6 Department of Chemistry, The University of Chicago, Chicago IL 60637, USA
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Aaron R. Dinner
1 James Franck Institute, The University of Chicago, Chicago IL 60637, USA
4 Institute for Biophysical Dynamics, The University of Chicago, Chicago IL 60637, USA
6 Department of Chemistry, The University of Chicago, Chicago IL 60637, USA
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  • For correspondence: dinner@uchicago.edu nfschere@uchicago.edu
Norbert F. Scherer
1 James Franck Institute, The University of Chicago, Chicago IL 60637, USA
4 Institute for Biophysical Dynamics, The University of Chicago, Chicago IL 60637, USA
6 Department of Chemistry, The University of Chicago, Chicago IL 60637, USA
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  • For correspondence: dinner@uchicago.edu nfschere@uchicago.edu
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Abstract

Cell size is specific to each species and impacts their ability to function. While various phenomenological models for cell size regulation have been proposed, recent work in bacteria have demonstrated an adder model, in which a cell increments its size by a constant amount between each division. However, the coupling between cell size, shape and constriction, remain poorly understood. Here, we investigate size control and the cell cycle dependence of bacterial growth, using multigenerational cell growth and shape data for single Caulobacter crescentus cells. Our analysis reveals a biphasic mode of growth: a relative timer phase before constriction where cell growth is correlated to its initial size, followed by a pure adder phase during constriction. Cell wall labeling measurements reinforce this biphasic model: a crossover from uniform lateral growth to localized septal growth is observed. We present a mathematical model that quantitatively explains this biphasic mixer model for cell size control.

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Posted June 26, 2017.
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Biphasic growth dynamics during Caulobacter crescentus division
Shiladitya Banerjee, Klevin Lo, Matthew K. Daddysman, Alan Selewa, Thomas Kuntz, Aaron R. Dinner, Norbert F. Scherer
bioRxiv 047589; doi: https://doi.org/10.1101/047589
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Biphasic growth dynamics during Caulobacter crescentus division
Shiladitya Banerjee, Klevin Lo, Matthew K. Daddysman, Alan Selewa, Thomas Kuntz, Aaron R. Dinner, Norbert F. Scherer
bioRxiv 047589; doi: https://doi.org/10.1101/047589

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