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A Transcriptional Lineage of the Early C. elegans Embryo

Sophia C. Tintori, Erin Osborne Nishimura, Patrick Golden, Jason D. Lieb, Bob Goldstein
doi: https://doi.org/10.1101/047746
Sophia C. Tintori
1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Erin Osborne Nishimura
1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
2Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523
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Patrick Golden
3School of Information and Library Science, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Jason D. Lieb
1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Bob Goldstein
1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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  • For correspondence: bobg@unc.edu
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Abstract

HIGHLIGHTS

  • ‒ RNA-seq on each cell of the early C. elegans embryo complements the known lineage

  • ‒ We measured the zygotic activation specific to each unique cell of the embryo

  • ‒ We identified genes that are functionally redundant and critical for development

  • ‒ We created an interactive online data visualization tool for exploring the data

eTOC BLURB C. elegans is a powerful model for development, with an invariant and completely described cell lineage. To enrich this resource, we performed single-cell RNA-seq on each cell of the embryo through the 16-cell stage. Zygotic genome activation is differential between cell types. We identified hundreds of candidates for partially redundant genes, and verified one such set as critical for development. We created an interactive online data visualization tool to invite others to explore our dataset.

SUMMARY During embryonic development, cells must establish fates, morphologies and behaviors in coordination with one another to form a functional body. A prevalent hypothesis for how this coordination is achieved is that each cell’s fate and behavior is determined by a defined mixture of RNAs. Only recently has it become possible to measure the full suite of transcripts in a single cell. Here we quantify the abundance of every mRNA transcript in each cell of the C. elegans embryo up to the 16-cell stage. We describe spatially dynamic expression, quantify cell-specific differential activation of the zygotic genome, and identify critical developmental genes previously unappreciated because of their partial redundancy. We present an interactive data visualization tool that allows broad access to our dataset. This genome-wide single-cell map of mRNA abundance, alongside the well-studied life history and fates of each cell, describes at a cellular resolution the mRNA landscape that guides development.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 08, 2016.
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A Transcriptional Lineage of the Early C. elegans Embryo
Sophia C. Tintori, Erin Osborne Nishimura, Patrick Golden, Jason D. Lieb, Bob Goldstein
bioRxiv 047746; doi: https://doi.org/10.1101/047746
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A Transcriptional Lineage of the Early C. elegans Embryo
Sophia C. Tintori, Erin Osborne Nishimura, Patrick Golden, Jason D. Lieb, Bob Goldstein
bioRxiv 047746; doi: https://doi.org/10.1101/047746

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