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Membrane binding by CHMP7 coordinates ESCRT-III dependent nuclear envelope reformation

Yolanda Olmos, Anna Perdrix, View ORCID ProfileJeremy G Carlton
doi: https://doi.org/10.1101/049221
Yolanda Olmos
1Division of Cancer Studies, Section of Cell Biology and Imaging, King’s College London, London SE1 1UL
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Anna Perdrix
1Division of Cancer Studies, Section of Cell Biology and Imaging, King’s College London, London SE1 1UL
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Jeremy G Carlton
1Division of Cancer Studies, Section of Cell Biology and Imaging, King’s College London, London SE1 1UL
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  • ORCID record for Jeremy G Carlton
  • For correspondence: jeremy.carlton@kcl.ac.uk
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Abstract

Amongst other cellular functions, the Endosomal Sorting Complex Required for Transport-III (ESCRT-III) machinery controls nuclear envelope (NE) reformation during mitotic exit by sealing holes in the reforming NE. ESCRT-III also acts to repair this organelle upon migration-induced rupture. The ESCRT-III component CHMP7 is responsible for recruitment of ESCRT-III to the NE. Here, we show that the N-terminus of CHMP7, comprising tandem Winged Helix (WH)-domains, is a membrane-binding module. This activity allows CHMP7 to bind to the Endoplasmic Reticulum (ER), an organelle continuous with the NE, and provides a platform to direct NE-recruitment of ESCRT-III during mitotic exit. Point mutations that disrupt membrane-binding prevent CHMP7 localising to the ER and its subsequent enrichment at the reforming NE. These mutations prevent both assembly of downstream ESCRT-III components at the reforming NE and proper establishment of post-mitotic nucleo-cytoplasmic compartmentalisation. These data identify a novel membrane-binding activity within an ESCRT-III subunit that is essential for post-mitotic nuclear regeneration.

One Sentence Summary CHMP7’s atypical N-terminus is a membrane-binding module that allows assembly and function of ESCRT-III at the nuclear envelope during mitotic exit.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted April 18, 2016.
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Membrane binding by CHMP7 coordinates ESCRT-III dependent nuclear envelope reformation
Yolanda Olmos, Anna Perdrix, Jeremy G Carlton
bioRxiv 049221; doi: https://doi.org/10.1101/049221
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Membrane binding by CHMP7 coordinates ESCRT-III dependent nuclear envelope reformation
Yolanda Olmos, Anna Perdrix, Jeremy G Carlton
bioRxiv 049221; doi: https://doi.org/10.1101/049221

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