Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The rate and effect of de novo mutations in natural populations of Arabidopsis thaliana

View ORCID ProfileMoises Exposito-Alonso, View ORCID ProfileClaude Becker, Verena J. Schuenemann, Ella Reitter, Claudia Setzer, Radka Slovak, Benjamin Brachi, Jöerg Hagmann, Dominik G. Grimm, Chen Jiahui, Wolfgang Busch, Joy Bergelson, Rob W. Ness, Johannes Krause, View ORCID ProfileHernán A. Burbano, View ORCID ProfileDetlef Weigel
doi: https://doi.org/10.1101/050203
Moises Exposito-Alonso
1Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
2Research Group for Ancient Genomics and Evolution, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
†Co-first authors
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Moises Exposito-Alonso
Claude Becker
1Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
†Co-first authors
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Claude Becker
Verena J. Schuenemann
3Institute of Archaeological Sciences, University of Tubingen, 72070 Tübingen, Germany
4Senckenberg Center for Human Evolution and Paleoenvironment, University of Tübingen, 72070 Tübingen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ella Reitter
3Institute of Archaeological Sciences, University of Tubingen, 72070 Tübingen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Claudia Setzer
5Gregor Mendel Institute, Austrian Academy of Sciences, 1030 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Radka Slovak
5Gregor Mendel Institute, Austrian Academy of Sciences, 1030 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin Brachi
6Department of Ecology and Evolution, University of Chicago, Chicago, Illinois 60637, USA
§Current addresses: INRA, UMR 1202 Biodiversité Gènes & Communautés, 69 route d’Arcachon, 33610 CESTAS, France (B.B.); Computomics, 72072 Tübingen, Germany (J.H.); Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland (D.G.G.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jöerg Hagmann
1Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
§Current addresses: INRA, UMR 1202 Biodiversité Gènes & Communautés, 69 route d’Arcachon, 33610 CESTAS, France (B.B.); Computomics, 72072 Tübingen, Germany (J.H.); Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland (D.G.G.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dominik G. Grimm
1Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
§Current addresses: INRA, UMR 1202 Biodiversité Gènes & Communautés, 69 route d’Arcachon, 33610 CESTAS, France (B.B.); Computomics, 72072 Tübingen, Germany (J.H.); Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland (D.G.G.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chen Jiahui
6Department of Ecology and Evolution, University of Chicago, Chicago, Illinois 60637, USA
7Institute of Tibet Plateau Research, Chinese Academy of Sciences, Beijing 100101, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wolfgang Busch
5Gregor Mendel Institute, Austrian Academy of Sciences, 1030 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joy Bergelson
6Department of Ecology and Evolution, University of Chicago, Chicago, Illinois 60637, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rob W. Ness
8Department of Biology, University of Toronto Mississauga, Mississauga, Ontario L5L IC6, Canada.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Johannes Krause
3Institute of Archaeological Sciences, University of Tubingen, 72070 Tübingen, Germany
4Senckenberg Center for Human Evolution and Paleoenvironment, University of Tübingen, 72070 Tübingen, Germany
9Max Planck Institute for the Science of Human History, 07743 Jena, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hernán A. Burbano
2Research Group for Ancient Genomics and Evolution, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hernán A. Burbano
  • For correspondence: weigel@weigelworld.org hernan.burbano@tuebingen.mpg.de
Detlef Weigel
1Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Detlef Weigel
  • For correspondence: weigel@weigelworld.org hernan.burbano@tuebingen.mpg.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

SUMMARY

Like many other species, the plant Arabidopsis thaliana has been introduced in recent history from its native Eurasian range to North America, with many individuals belonging to a single lineage. We have sequenced 100 genomes of present-day and herbarium specimens from this lineage, covering the time span from 1863 to 2006. Within-lineage recombination was nearly absent, greatly simplifying the genetic analysis, allowing direct estimation of the mutation rate and an introduction date in the early-17th century. The comparison of substitution rates at different sites throughout the genome reveals that genetic drift predominates, but that purifying selection in this rapidly expanding population is nevertheless evident even over short historical time scales. Furthermore, an association analysis identifies new mutations affecting root development, a trait important for adaptation in the wild. Our work illustrates how mutation and selection rates can be observed directly by combining modern genetic methods and historic samples.

HIGHLIGHTS

  • A historically young colonizing lineage of Arabidopsis thaliana allows observation of contemporary evolutionary forces.

  • Genomes from specimens collected over 150 years support direct calculation of mutation rates occurring in nature.

  • Drift predominates, but purifying selection is evident genome-wide over historical time scales.

  • New mutations with phenotypic effects can be identified and traced back in time and space.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted April 25, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The rate and effect of de novo mutations in natural populations of Arabidopsis thaliana
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The rate and effect of de novo mutations in natural populations of Arabidopsis thaliana
Moises Exposito-Alonso, Claude Becker, Verena J. Schuenemann, Ella Reitter, Claudia Setzer, Radka Slovak, Benjamin Brachi, Jöerg Hagmann, Dominik G. Grimm, Chen Jiahui, Wolfgang Busch, Joy Bergelson, Rob W. Ness, Johannes Krause, Hernán A. Burbano, Detlef Weigel
bioRxiv 050203; doi: https://doi.org/10.1101/050203
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
The rate and effect of de novo mutations in natural populations of Arabidopsis thaliana
Moises Exposito-Alonso, Claude Becker, Verena J. Schuenemann, Ella Reitter, Claudia Setzer, Radka Slovak, Benjamin Brachi, Jöerg Hagmann, Dominik G. Grimm, Chen Jiahui, Wolfgang Busch, Joy Bergelson, Rob W. Ness, Johannes Krause, Hernán A. Burbano, Detlef Weigel
bioRxiv 050203; doi: https://doi.org/10.1101/050203

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4087)
  • Biochemistry (8766)
  • Bioengineering (6480)
  • Bioinformatics (23346)
  • Biophysics (11751)
  • Cancer Biology (9149)
  • Cell Biology (13255)
  • Clinical Trials (138)
  • Developmental Biology (7417)
  • Ecology (11369)
  • Epidemiology (2066)
  • Evolutionary Biology (15088)
  • Genetics (10402)
  • Genomics (14011)
  • Immunology (9122)
  • Microbiology (22050)
  • Molecular Biology (8780)
  • Neuroscience (47373)
  • Paleontology (350)
  • Pathology (1420)
  • Pharmacology and Toxicology (2482)
  • Physiology (3704)
  • Plant Biology (8050)
  • Scientific Communication and Education (1431)
  • Synthetic Biology (2209)
  • Systems Biology (6016)
  • Zoology (1250)