Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Pan-retinal characterization of Light Responses from Ganglion Cells in the Developing Mouse Retina

View ORCID ProfileGerrit Hilgen, View ORCID ProfileSahar Pirmoradian, Daniela Pamplona, Pierre Kornprobst, Bruno Cessac, View ORCID ProfileMatthias H. Hennig, View ORCID ProfileEvelyne Sernagor
doi: https://doi.org/10.1101/050393
Gerrit Hilgen
1Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gerrit Hilgen
Sahar Pirmoradian
2Institute for Adaptive and Neural Computation, University of Edinburgh EH8 9AB, Edinburgh, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sahar Pirmoradian
Daniela Pamplona
3Inria, Neuromathcomp Team, 06902 Sophia Antipolis, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pierre Kornprobst
3Inria, Neuromathcomp Team, 06902 Sophia Antipolis, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bruno Cessac
3Inria, Neuromathcomp Team, 06902 Sophia Antipolis, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthias H. Hennig
2Institute for Adaptive and Neural Computation, University of Edinburgh EH8 9AB, Edinburgh, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Matthias H. Hennig
Evelyne Sernagor
1Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Evelyne Sernagor
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The functional organization of the mammalian retina is not spatially uniform. These functional inhomogenities presumably represent adaptations to specific visual needs associated with particular ecological benefits. However, how such asymmetries develop has not been documented yet. Here we present the ontogeny of light responses in mouse RGCs from eye opening to adulthood. Using a large-scale, high-density multielectrode array, we were able to record simultaneously from hundreds to thousands of dorsal and ventral RGCs, documenting light response properties (firing peak amplitude, time to peak and response duration) for the three major RGC types (ON, OFF, ON-OFF). Responses to different contrasts not only revealed a complex developmental profile for all three RGC types, but also unveiled differences between RGCs located in the dorsal versus ventral retina. At eye-opening, dorsal RGCs of all types were significantly more responsive to light, perhaps indicating an ecological advantage to nest viewing for young, pre-weaning pups. At the same time, the developmental profile of ON and OFF RGCs exhibited antagonistic behavior, with the strongest ON responses shortly after eye-opening, followed by an increase in the strength of OFF responses at later stages. We estimated RGC receptive field sizes and polarity across development using a novel super-resolution approach based on white noise and spike-triggered average (STA) responses and found that receptive field centers decrease but STA strength increases with maturation. These results show for the first time that the refinement of developing RGC responses to light is also not spatially uniform across the retina.

Significance Statement (120 maximum) Using a large-scale, high-density multielectrode array, we have investigated the ontogeny of light responses in retinal ganglion cells (RGCs) in the developing mouse retina. We were able to record from hundreds to thousands of RGCs simultaneously at pan-retinal level, and found that the refinement of receptive field properties strikingly differs between RGCs in the dorsal and ventral retina, with stronger ON and generally more prominent responses in the dorsal retina immediately after eye opening, followed by an increase in OFF responses. These findings suggest that retinal functionality is not spatially uniform and that there might be an ecological advantage to favoring the development of dorsal light responses before the rest of the retina reaches functional maturity.

Conflict of Interest The authors declare no competing financial interests.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted April 26, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Pan-retinal characterization of Light Responses from Ganglion Cells in the Developing Mouse Retina
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Pan-retinal characterization of Light Responses from Ganglion Cells in the Developing Mouse Retina
Gerrit Hilgen, Sahar Pirmoradian, Daniela Pamplona, Pierre Kornprobst, Bruno Cessac, Matthias H. Hennig, Evelyne Sernagor
bioRxiv 050393; doi: https://doi.org/10.1101/050393
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Pan-retinal characterization of Light Responses from Ganglion Cells in the Developing Mouse Retina
Gerrit Hilgen, Sahar Pirmoradian, Daniela Pamplona, Pierre Kornprobst, Bruno Cessac, Matthias H. Hennig, Evelyne Sernagor
bioRxiv 050393; doi: https://doi.org/10.1101/050393

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Subject Areas
All Articles
  • Animal Behavior and Cognition (4095)
  • Biochemistry (8789)
  • Bioengineering (6494)
  • Bioinformatics (23403)
  • Biophysics (11767)
  • Cancer Biology (9172)
  • Cell Biology (13294)
  • Clinical Trials (138)
  • Developmental Biology (7423)
  • Ecology (11391)
  • Epidemiology (2066)
  • Evolutionary Biology (15123)
  • Genetics (10418)
  • Genomics (14028)
  • Immunology (9154)
  • Microbiology (22129)
  • Molecular Biology (8796)
  • Neuroscience (47464)
  • Paleontology (350)
  • Pathology (1423)
  • Pharmacology and Toxicology (2486)
  • Physiology (3712)
  • Plant Biology (8069)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2216)
  • Systems Biology (6022)
  • Zoology (1251)