Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

QTL mapping reveals complex genetic architecture of quantitative virulence in the wheat pathogen Zymoseptoria tritici

E. L. Stewart, D. Croll, M. H. Lendenmann, A. Sanchez Vallet, F. E. Hartmann, J Palma Guerrero, Z. Ma, B. A. McDonald
doi: https://doi.org/10.1101/051169
E. L. Stewart
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D. Croll
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. H. Lendenmann
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. Sanchez Vallet
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F. E. Hartmann
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Palma Guerrero
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Z. Ma
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
B. A. McDonald
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Summary

We conducted a comprehensive analysis of virulence in the fungal wheat pathogen Zymoseptoria tritici using QTL mapping. High throughput phenotyping based on automated image analysis allowed measurement of pathogen virulence on a scale and with a precision that was not previously possible. Across two mapping populations encompassing more than 520 progeny, 540,710 pycnidia were counted and their sizes and grey values were measured, yielding over 1.6 million phenotypes associated with pathogen reproduction. Large pycnidia were shown to produce more numerous and larger spores than small pycnidia. Precise measures of percent leaf area covered by lesions provided a quantitative measure of host damage. Combining these large and accurate phenotype datasets with a dense panel of RADseq genetic markers enabled us to genetically dissect pathogen virulence into components related to host damage and components related to pathogen reproduction. We show that different components of virulence can be under separate genetic control. Large-and small-effect QTLs were identified for all traits, with some QTLs specific to mapping populations, cultivars and traits and other QTLs shared among traits within the same mapping population. We associated the presence or absence of accessory chromosomes with several virulence traits, providing the first evidence for an important function associated with accessory chromosomes in this organism. A large-effect QTL involved in host specialization was identified on chromosome 7, leading to identification of candidate genes having a large effect on virulence.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted May 07, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
QTL mapping reveals complex genetic architecture of quantitative virulence in the wheat pathogen Zymoseptoria tritici
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
QTL mapping reveals complex genetic architecture of quantitative virulence in the wheat pathogen Zymoseptoria tritici
E. L. Stewart, D. Croll, M. H. Lendenmann, A. Sanchez Vallet, F. E. Hartmann, J Palma Guerrero, Z. Ma, B. A. McDonald
bioRxiv 051169; doi: https://doi.org/10.1101/051169
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
QTL mapping reveals complex genetic architecture of quantitative virulence in the wheat pathogen Zymoseptoria tritici
E. L. Stewart, D. Croll, M. H. Lendenmann, A. Sanchez Vallet, F. E. Hartmann, J Palma Guerrero, Z. Ma, B. A. McDonald
bioRxiv 051169; doi: https://doi.org/10.1101/051169

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genetics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3502)
  • Biochemistry (7343)
  • Bioengineering (5319)
  • Bioinformatics (20258)
  • Biophysics (10008)
  • Cancer Biology (7735)
  • Cell Biology (11293)
  • Clinical Trials (138)
  • Developmental Biology (6434)
  • Ecology (9947)
  • Epidemiology (2065)
  • Evolutionary Biology (13315)
  • Genetics (9359)
  • Genomics (12579)
  • Immunology (7696)
  • Microbiology (19008)
  • Molecular Biology (7437)
  • Neuroscience (41011)
  • Paleontology (300)
  • Pathology (1228)
  • Pharmacology and Toxicology (2134)
  • Physiology (3155)
  • Plant Biology (6858)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1895)
  • Systems Biology (5311)
  • Zoology (1087)