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Calcium imaging reveals coordinated simple spike pauses in populations of Cerebellar Purkinje cells

View ORCID ProfileJorge E. Ramirez, View ORCID ProfileBrandon M. Stell
doi: https://doi.org/10.1101/051730
Jorge E. Ramirez
CNRS, University of Paris Descartes, Paris, France
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Brandon M. Stell
CNRS, University of Paris Descartes, Paris, France
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Abstract

The brain’s control of movement is thought to involve coordinated activity between cerebellar Purkinje cells. The results reported here demonstrate that somatic Ca2+ imaging is a faithful reporter of Na+-dependent “simple spike” pauses and enables us to optically record changes in firing rates in populations of Purkinje cells. This simultaneous calcium imaging of populations of Purkinje cells reveals a striking spatial organization of pauses in Purkinje cell activity between neighboring cells. The source of this organization is shown to be the presynaptic GABAergic network and blocking GABAARs abolishes the synchrony. These data suggest that presynaptic interneurons synchronize (in)activity between neighboring Purkinje cells and thereby maximize their effect on downstream targets in the deep cerebellar nuclei.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted May 04, 2016.
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Calcium imaging reveals coordinated simple spike pauses in populations of Cerebellar Purkinje cells
Jorge E. Ramirez, Brandon M. Stell
bioRxiv 051730; doi: https://doi.org/10.1101/051730
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Calcium imaging reveals coordinated simple spike pauses in populations of Cerebellar Purkinje cells
Jorge E. Ramirez, Brandon M. Stell
bioRxiv 051730; doi: https://doi.org/10.1101/051730

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