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Synaptonemal complex components are required for meiotic checkpoint function in C. elegans

Tisha Bohr, Guinevere Ashley, Evan Eggleston, Kyra Firestone, Needhi Bhalla
doi: https://doi.org/10.1101/052977
Tisha Bohr
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064
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Guinevere Ashley
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064
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Evan Eggleston
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064
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Kyra Firestone
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064
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Needhi Bhalla
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064
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  • For correspondence: nbhalla@ucsc.edu
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Abstract

Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes and is essential for proper meiotic chromosome segregation. In C. elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on Pairing Centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3 and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bonafide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 01, 2016.
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Synaptonemal complex components are required for meiotic checkpoint function in C. elegans
Tisha Bohr, Guinevere Ashley, Evan Eggleston, Kyra Firestone, Needhi Bhalla
bioRxiv 052977; doi: https://doi.org/10.1101/052977
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Synaptonemal complex components are required for meiotic checkpoint function in C. elegans
Tisha Bohr, Guinevere Ashley, Evan Eggleston, Kyra Firestone, Needhi Bhalla
bioRxiv 052977; doi: https://doi.org/10.1101/052977

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