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Genome sequencing of a single tardigrade Hypsibius dujardini individual

View ORCID ProfileKazuharu Arakawa, Yuki Yoshida, Masaru Tomita
doi: https://doi.org/10.1101/053223
Kazuharu Arakawa
1Institute for Advanced Biosciences, Keio University
2Systems Biology Program, Graduate School of Media and Governance, Keio University
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  • ORCID record for Kazuharu Arakawa
  • For correspondence: gaou@sfc.keio.ac.jp
Yuki Yoshida
1Institute for Advanced Biosciences, Keio University
2Systems Biology Program, Graduate School of Media and Governance, Keio University
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Masaru Tomita
1Institute for Advanced Biosciences, Keio University
2Systems Biology Program, Graduate School of Media and Governance, Keio University
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Abstract

Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 13, 2016.
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Genome sequencing of a single tardigrade Hypsibius dujardini individual
Kazuharu Arakawa, Yuki Yoshida, Masaru Tomita
bioRxiv 053223; doi: https://doi.org/10.1101/053223
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Genome sequencing of a single tardigrade Hypsibius dujardini individual
Kazuharu Arakawa, Yuki Yoshida, Masaru Tomita
bioRxiv 053223; doi: https://doi.org/10.1101/053223

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