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50-valent inactivated rhinovirus vaccine is broadly immunogenic in rhesus macaques

Sujin Lee, Minh Trang Nguyen, Michael G. Currier, Joe B. Jenkins, Elizabeth A. Strobert, Adriana E. Kajon, Ranjna Madan-Lala, Yury A. Bochkov, James E. Gern, Krishnendu Roy, Xiaoyan Lu, Dean D. Erdman, Paul Spearman, Martin L. Moore
doi: https://doi.org/10.1101/053967
Sujin Lee
1Department of Pediatrics, Emory University, Atlanta, Georgia 30322, USA.
2Children’s Healthcare of Atlanta, Atlanta, Georgia 30322, USA.
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Minh Trang Nguyen
1Department of Pediatrics, Emory University, Atlanta, Georgia 30322, USA.
2Children’s Healthcare of Atlanta, Atlanta, Georgia 30322, USA.
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Michael G. Currier
1Department of Pediatrics, Emory University, Atlanta, Georgia 30322, USA.
2Children’s Healthcare of Atlanta, Atlanta, Georgia 30322, USA.
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Joe B. Jenkins
3Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329.
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Elizabeth A. Strobert
3Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329.
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Adriana E. Kajon
4Infectious Disease Program, Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87108, USA.
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Ranjna Madan-Lala
5The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30322, USA.
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Yury A. Bochkov
6Department of Pediatrics
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James E. Gern
6Department of Pediatrics
7Department of Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53792, USA.
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Krishnendu Roy
5The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30322, USA.
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Xiaoyan Lu
8Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
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Dean D. Erdman
8Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
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Paul Spearman
1Department of Pediatrics, Emory University, Atlanta, Georgia 30322, USA.
2Children’s Healthcare of Atlanta, Atlanta, Georgia 30322, USA.
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Martin L. Moore
1Department of Pediatrics, Emory University, Atlanta, Georgia 30322, USA.
2Children’s Healthcare of Atlanta, Atlanta, Georgia 30322, USA.
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  • For correspondence: martin.moore@emory.edu
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Abstract

As the predominant etiological agent of the common cold, human rhinovirus (HRV) is the leading cause of human infectious disease. Early studies showed monovalent formalin-inactivated HRV vaccine can be protective, and virus-neutralizing antibodies (nAb) correlated with protection. However, co-circulation of many HRV types discouraged further vaccine efforts. We approached this problem straightforwardly. We tested the hypothesis that increasing virus input titers in polyvalent inactivated HRV vaccine will result in broad nAb responses. Here, we show that serum nAb against many rhinovirus types can be induced by polyvalent, inactivated HRVs plus alhydrogel (alum) adjuvant. Using formulations up to 25-valent in mice and 50-valent in rhesus macaques, HRV vaccine immunogenicity was related to sufficient quantity of input antigens, and valency was not a major factor for potency or breadth of the response. We for the first time generated a vaccine capable of inducing nAb responses to numerous and diverse HRV types.

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Posted May 17, 2016.
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50-valent inactivated rhinovirus vaccine is broadly immunogenic in rhesus macaques
Sujin Lee, Minh Trang Nguyen, Michael G. Currier, Joe B. Jenkins, Elizabeth A. Strobert, Adriana E. Kajon, Ranjna Madan-Lala, Yury A. Bochkov, James E. Gern, Krishnendu Roy, Xiaoyan Lu, Dean D. Erdman, Paul Spearman, Martin L. Moore
bioRxiv 053967; doi: https://doi.org/10.1101/053967
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50-valent inactivated rhinovirus vaccine is broadly immunogenic in rhesus macaques
Sujin Lee, Minh Trang Nguyen, Michael G. Currier, Joe B. Jenkins, Elizabeth A. Strobert, Adriana E. Kajon, Ranjna Madan-Lala, Yury A. Bochkov, James E. Gern, Krishnendu Roy, Xiaoyan Lu, Dean D. Erdman, Paul Spearman, Martin L. Moore
bioRxiv 053967; doi: https://doi.org/10.1101/053967

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