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Parent-of-origin effect rough endosperm mutants in maize

Fang Bai, Mary Daliberti, Alyssa Bagadion, Miaoyun Xu, Yubing Li, John Baier, Chi Wah Tseung, Matthew M. S. Evans, A. Mark Settles
doi: https://doi.org/10.1101/054338
Fang Bai
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611
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Mary Daliberti
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611
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Alyssa Bagadion
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611
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Miaoyun Xu
Biotechnology Research Institute, National Key Facility for Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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Yubing Li
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611
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John Baier
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611
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Chi Wah Tseung
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611
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Matthew M. S. Evans
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA
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A. Mark Settles
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611
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ABSTRACT

Parent-of-origin effect loci have non-Mendelian inheritance in which phenotypes are determined by either the maternal or paternal allele alone. In angiosperms, parent-of-origin effects can be caused by loci required for gametophyte development or by imprinted genes needed for seed development. Few parent-of-origin effect loci have been identified in maize (Zea mays) even though there are a large number of imprinted genes known from transcriptomics. We screened rough endosperm (rgh) mutants for parent-of-origin effects using reciprocal crosses with inbred parents. Six maternal rough endosperm (mre) and three paternal rough endosperm (pre) mutants were identified with three mre loci mapped. When inherited from the female parent, mre/+ seeds reduce grain-fill with a rough, etched, or pitted endosperm surface. Pollen transmission of pre mutants results in rgh endosperm as well as embryo lethality. Eight of the loci had significant distortion from the expected one-to-one ratio for parent-of-origin effects. Linked markers for mre1, mre2, and mre3 indicated that the mutant alleles have no bias in transmission. Histological analysis of mre1, mre2, mre3, and pre*-949 showed altered timing of starch grain accumulation and basal endosperm transfer cell layer (BETL) development. The mre1 locus delays BETL and starchy endosperm development, while mre2 and pre*-949 cause ectopic starchy endosperm differentiation. We conclude that many parent-of-origin effects in maize have incomplete penetrance of kernel phenotypes and that there is a large diversity of endosperm developmental roles for parent-of-origin effect loci.

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Posted May 19, 2016.
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Parent-of-origin effect rough endosperm mutants in maize
Fang Bai, Mary Daliberti, Alyssa Bagadion, Miaoyun Xu, Yubing Li, John Baier, Chi Wah Tseung, Matthew M. S. Evans, A. Mark Settles
bioRxiv 054338; doi: https://doi.org/10.1101/054338
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Parent-of-origin effect rough endosperm mutants in maize
Fang Bai, Mary Daliberti, Alyssa Bagadion, Miaoyun Xu, Yubing Li, John Baier, Chi Wah Tseung, Matthew M. S. Evans, A. Mark Settles
bioRxiv 054338; doi: https://doi.org/10.1101/054338

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