Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A Facilitated Diffusion Mechanism Establishes the Drosophila Dorsal Gradient

Sophia N. Carrell, Michael D. O’Connell, Amy E. Allen, Stephanie M. Smith, Gregory T. Reeves
doi: https://doi.org/10.1101/057091
Sophia N. Carrell
1Department of Chemical & Biomolecular Engineering, North Carolina State University,Raleigh, NC 27605,USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael D. O’Connell
1Department of Chemical & Biomolecular Engineering, North Carolina State University,Raleigh, NC 27605,USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amy E. Allen
1Department of Chemical & Biomolecular Engineering, North Carolina State University,Raleigh, NC 27605,USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephanie M. Smith
1Department of Chemical & Biomolecular Engineering, North Carolina State University,Raleigh, NC 27605,USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gregory T. Reeves
1Department of Chemical & Biomolecular Engineering, North Carolina State University,Raleigh, NC 27605,USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: gtreeves@ncsu.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Summary

The transcription factor NF-κB plays an important role in the immune system as an apoptotic and inflammatory factor. In the Drosophila melanogaster embryo, a homolog of NF-ΚB called Dorsal (dl) patterns the dorsal-ventral (DV) axis in a concentration-dependent manner. During early development, dl is sequestered outside the nucleus by Cactus (Cact), homologous to IκB. Toll signaling at the ventral midline breaks the dl/Cact complex, allowing dl to enter the nucleus where it transcribes target genes. Here we show that dl accumulates on the ventral side of the embryo over the last 5 cleavage cycles and that this accumulation is the result of facilitated diffusion of dl/Cact complex. We speculate that the predominant role for Cact in DV axis specification is to shuttle dl towards the ventral midline. Given that this mechanism has been found in other, independent systems, we suggest it may be more prevalent than previously thought.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted June 03, 2016.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A Facilitated Diffusion Mechanism Establishes the Drosophila Dorsal Gradient
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A Facilitated Diffusion Mechanism Establishes the Drosophila Dorsal Gradient
Sophia N. Carrell, Michael D. O’Connell, Amy E. Allen, Stephanie M. Smith, Gregory T. Reeves
bioRxiv 057091; doi: https://doi.org/10.1101/057091
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
A Facilitated Diffusion Mechanism Establishes the Drosophila Dorsal Gradient
Sophia N. Carrell, Michael D. O’Connell, Amy E. Allen, Stephanie M. Smith, Gregory T. Reeves
bioRxiv 057091; doi: https://doi.org/10.1101/057091

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4115)
  • Biochemistry (8818)
  • Bioengineering (6522)
  • Bioinformatics (23466)
  • Biophysics (11792)
  • Cancer Biology (9212)
  • Cell Biology (13326)
  • Clinical Trials (138)
  • Developmental Biology (7439)
  • Ecology (11413)
  • Epidemiology (2066)
  • Evolutionary Biology (15155)
  • Genetics (10439)
  • Genomics (14045)
  • Immunology (9173)
  • Microbiology (22159)
  • Molecular Biology (8814)
  • Neuroscience (47581)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3731)
  • Plant Biology (8082)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1253)