Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
Confirmatory Results

Tandem duplication events in the expansion of the small heat shock protein gene family in Solanum lycopersicum (cv. Heinz 1706)

Flavia J. Krsticevic, Débora P. Arce, Joaquín Ezpeleta, Elizabeth Tapia
doi: https://doi.org/10.1101/057182
Flavia J. Krsticevic
*CIFASIS, Centro InternacionalFranco Argentino de Ciencias de la Información y de Sistemas - CONICET - UNR - AMU, Rosario, Argentina
†GADIB, Grupode Análisis, Desarrollos e Investigaciones Biomédicas, Facultad Regional San Nicolás, Universidad Tecnológica Nacional, San Nicols, Argentina,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Débora P. Arce
†GADIB, Grupode Análisis, Desarrollos e Investigaciones Biomédicas, Facultad Regional San Nicolás, Universidad Tecnológica Nacional, San Nicols, Argentina,
‡Cátedrade Genética, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joaquín Ezpeleta
*CIFASIS, Centro InternacionalFranco Argentino de Ciencias de la Información y de Sistemas - CONICET - UNR - AMU, Rosario, Argentina
§Facultad de Ciencias Exactas, Ingeniería y Agrimensura, Universidad Nacional de Rosario, Rosario, Argentina
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth Tapia
*CIFASIS, Centro InternacionalFranco Argentino de Ciencias de la Información y de Sistemas - CONICET - UNR - AMU, Rosario, Argentina
§Facultad de Ciencias Exactas, Ingeniería y Agrimensura, Universidad Nacional de Rosario, Rosario, Argentina
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

In plants, fruit maturation and oxidative stress can induce small heat shock protein (sHSP) synthesis to maintain cellular homeostasis. Although the tomato reference genome was published in 2012, the actual number and functionality of sHSP genes remain unknown. Using a transcriptomic (RNA-seq) and evolutionary genomic approach, putative sHSP genes in the Solanum lycopersicum (cv. Heinz 1706) genome are investigated. A sHSP gene family of 33 members is established. Remarkably, roughly half of themembers of this family can be explained by nine independent tandem duplication eventsthat evolutionarily determined their functional fates. Within a mitochondrial class subfamily, only one duplicated member, Solyc08g078700, retained its ancestral chaperonefunction, while the others, Solyc08g078710 and Solyc08g078720,likely degenerated under neutrality and lack ancestral chaperone function. Functional conservation occurred within a cytosolic class I subfamily, whose four members, Solyc06g076570, Solyc06g076560, Solyc06g076540 and Solyc06g076520, support ~57% of the total sHSP RNAm in the red ripe fruit. Sub-functionalization occurred within a new subfamily, whose twomembers, Solyc04g082720 and Solyc04g082740, show heterogeneous differential expressionprofiles during fruit ripening. These findings, involving the birth/death of some genes or the preferential/plastic expression of some others during fruit ripening, highlight the importance of tandem duplication events in the expansion of the sHSP gene family in the tomato genome. Despite its evolutionary diversity, the sHSP gene family in the tomato genome seems to be endowed with a core set of four homeostasis genes: Solyc05g014280, Solyc03g082420, Solyc11g020330 and Solyc06g076560, which appear to provide abaseline protection during both fruit ripening and heat shock stress in different tomato tissues.

Footnotes

  • ↵1 Ocampo y Esmeralda. 2000EZP Rosario. krsticevic{at}cifasis-conicet.gov.ar

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted June 09, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Tandem duplication events in the expansion of the small heat shock protein gene family in Solanum lycopersicum (cv. Heinz 1706)
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Tandem duplication events in the expansion of the small heat shock protein gene family in Solanum lycopersicum (cv. Heinz 1706)
Flavia J. Krsticevic, Débora P. Arce, Joaquín Ezpeleta, Elizabeth Tapia
bioRxiv 057182; doi: https://doi.org/10.1101/057182
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Tandem duplication events in the expansion of the small heat shock protein gene family in Solanum lycopersicum (cv. Heinz 1706)
Flavia J. Krsticevic, Débora P. Arce, Joaquín Ezpeleta, Elizabeth Tapia
bioRxiv 057182; doi: https://doi.org/10.1101/057182

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Evolutionary Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3602)
  • Biochemistry (7568)
  • Bioengineering (5523)
  • Bioinformatics (20789)
  • Biophysics (10327)
  • Cancer Biology (7980)
  • Cell Biology (11638)
  • Clinical Trials (138)
  • Developmental Biology (6602)
  • Ecology (10202)
  • Epidemiology (2065)
  • Evolutionary Biology (13615)
  • Genetics (9541)
  • Genomics (12846)
  • Immunology (7920)
  • Microbiology (19541)
  • Molecular Biology (7657)
  • Neuroscience (42093)
  • Paleontology (308)
  • Pathology (1258)
  • Pharmacology and Toxicology (2202)
  • Physiology (3267)
  • Plant Biology (7040)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1951)
  • Systems Biology (5426)
  • Zoology (1117)