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Vibrio natriegens, a new genomic powerhouse

Henry H. Lee, Nili Ostrov, Brandon G. Wong, Michaela A. Gold, Ahmad S. Khalil, George M. Church
doi: https://doi.org/10.1101/058487
Henry H. Lee
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
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Nili Ostrov
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
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Brandon G. Wong
2Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, Massachusetts 02215, USA.
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Michaela A. Gold
3Department of Biology, Chemical and Biological Engineering, Tufts University, Medford, Massachusetts, 02155.
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Ahmad S. Khalil
2Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, Massachusetts 02215, USA.
4Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.
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George M. Church
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
4Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.
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Abstract

Recombinant DNA technology has revolutionized biomedical research with continual innovations advancing the speed and throughput of molecular biology. Nearly all these tools, however, are reliant on Escherichia coli as a host organism, and its lengthy growth rate increasingly dominates experimental time. Here we report the development of Vibrio natriegens, a free-living bacteria with the fastest generation time known, into a genetically tractable host organism. We systematically characterize its growth properties to establish basic laboratory culturing conditions. We provide the first complete Vibrio natriegens genome, consisting of two chromosomes of 3,248,023 bp and 1,927,310 bp that together encode 4,578 open reading frames. We reveal genetic tools and techniques for working with Vibrio natriegens. These foundational resources will usher in an era of advanced genomics to accelerate biological, biotechnological, and medical discoveries.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 12, 2016.
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Vibrio natriegens, a new genomic powerhouse
Henry H. Lee, Nili Ostrov, Brandon G. Wong, Michaela A. Gold, Ahmad S. Khalil, George M. Church
bioRxiv 058487; doi: https://doi.org/10.1101/058487
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Vibrio natriegens, a new genomic powerhouse
Henry H. Lee, Nili Ostrov, Brandon G. Wong, Michaela A. Gold, Ahmad S. Khalil, George M. Church
bioRxiv 058487; doi: https://doi.org/10.1101/058487

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