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Metabarcoding of unfractionated water samples relates phyto-, zoo- and bacterioplankton dynamics and reveals a single-taxon bacterial bloom

Christian Wurzbacher, Katrin Attermeyer, Marie Therese Kettner, Clara Flintrop, Norman Warthmann, Sabine Hilt, Hans-Peter Grossart, Michael T. Monaghan
doi: https://doi.org/10.1101/058628
Christian Wurzbacher
1University of Gothenburg, Department of Biological and Environmental Sciences, Box 461, 40530 Göteborg, Sweden
2Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany
3Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775 Stechlin, Germany
4Berlin Center for Genomics in Biodiversity Research, Königin-Luise-Str. 6-8, 14195 Berlin, Germany
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  • For correspondence: christian@wurzbacher.cc katrin.attermeyer@ebc.uu.se
Katrin Attermeyer
3Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775 Stechlin, Germany
5Uppsala University, Department of Ecology and Genetics, Limnology, Norbyvägen 18D, 75236 Uppsala, Sweden
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  • For correspondence: christian@wurzbacher.cc katrin.attermeyer@ebc.uu.se
Marie Therese Kettner
3Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775 Stechlin, Germany
6Potsdam University, Institute of Biochemistry and Biology, Maulbeerallee 2, 14469 Potsdam, Germany
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Clara Flintrop
4Berlin Center for Genomics in Biodiversity Research, Königin-Luise-Str. 6-8, 14195 Berlin, Germany
7Helmholtz Young Investigator Group SEAPUMP, Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, and MARUM, University of Bremen, Leobener Strasse, 28359 Bremen, Germany
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Norman Warthmann
2Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany
4Berlin Center for Genomics in Biodiversity Research, Königin-Luise-Str. 6-8, 14195 Berlin, Germany
8The Australian National University, Research School of Biology, Linnaeus Way, Canberra, ACT 2601, Australia
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Sabine Hilt
2Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany
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Hans-Peter Grossart
3Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775 Stechlin, Germany
6Potsdam University, Institute of Biochemistry and Biology, Maulbeerallee 2, 14469 Potsdam, Germany
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Michael T. Monaghan
2Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany
4Berlin Center for Genomics in Biodiversity Research, Königin-Luise-Str. 6-8, 14195 Berlin, Germany
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Summary

Most studies of aquatic plankton focus on either macroscopic or microbial communities, and on either eukaryotes or prokaryotes. This separation is primarily for methodological reasons, but can overlook potential interactions among groups. We tested whether DNA-metabarcoding of unfractionated water samples with universal primers could be used to qualitatively and quantitatively study the temporal dynamics of the total plankton community in a shallow temperate lake. We found significant changes in the relative proportions of normalized sequence reads of eukaryotic and prokaryotic plankton communities over a three-month period in spring. Patterns followed the same trend as plankton estimates using traditional microscopic methods. We characterized the bloom of a conditionally rare bacterial taxon belonging to Arcicella, which rapidly came to dominate the whole lake ecosystem and would have remained unnoticed without metabarcoding. Our data demonstrate the potential of universal DNA-metabarcoding applied to unfractionated samples for providing a more holistic view of plankton communities.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 21, 2017.
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Metabarcoding of unfractionated water samples relates phyto-, zoo- and bacterioplankton dynamics and reveals a single-taxon bacterial bloom
Christian Wurzbacher, Katrin Attermeyer, Marie Therese Kettner, Clara Flintrop, Norman Warthmann, Sabine Hilt, Hans-Peter Grossart, Michael T. Monaghan
bioRxiv 058628; doi: https://doi.org/10.1101/058628
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Metabarcoding of unfractionated water samples relates phyto-, zoo- and bacterioplankton dynamics and reveals a single-taxon bacterial bloom
Christian Wurzbacher, Katrin Attermeyer, Marie Therese Kettner, Clara Flintrop, Norman Warthmann, Sabine Hilt, Hans-Peter Grossart, Michael T. Monaghan
bioRxiv 058628; doi: https://doi.org/10.1101/058628

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