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Anisotropic Correction of Beam-induced Motion for Improved Single-particle Electron Cryo-microscopy

Shawn Q. Zheng, Eugene Palovcak, Jean-Paul Armache, Yifan Cheng, David A. Agard
doi: https://doi.org/10.1101/061960
Shawn Q. Zheng
1Department of Biochemistry and Biophysics
2Howard Hughes Medical Institute, University of California, San Francisco, CA 94158
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Eugene Palovcak
1Department of Biochemistry and Biophysics
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Jean-Paul Armache
1Department of Biochemistry and Biophysics
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Yifan Cheng
1Department of Biochemistry and Biophysics
2Howard Hughes Medical Institute, University of California, San Francisco, CA 94158
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David A. Agard
1Department of Biochemistry and Biophysics
2Howard Hughes Medical Institute, University of California, San Francisco, CA 94158
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Abstract

Correction of electron beam-induced sample motion is one of the major factors contributing to the recent resolution breakthroughs in cryo-electron microscopy. Improving the accuracy and efficiency of motion correction can lead to further resolution improvement. Based on observations that the electron beam induces doming of the thin vitreous ice layer, we developed an algorithm to correct anisotropic image motion at the single pixel level across the whole frame, suitable for both single particle and tomographic images. Iterative, patch-based motion detection is combined with spatial and temporal constraints and dose weighting. The multi-GPU accelerated program, MotionCor2, is sufficiently fast to keep up with automated data collection. The result is an exceptionally robust strategy that can work on a wide range of data sets, including those very close to focus or with very short integration times, obviating the need for particle polishing. Application significantly improves Thon ring quality and 3D reconstruction resolution.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 04, 2016.
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Anisotropic Correction of Beam-induced Motion for Improved Single-particle Electron Cryo-microscopy
Shawn Q. Zheng, Eugene Palovcak, Jean-Paul Armache, Yifan Cheng, David A. Agard
bioRxiv 061960; doi: https://doi.org/10.1101/061960
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Anisotropic Correction of Beam-induced Motion for Improved Single-particle Electron Cryo-microscopy
Shawn Q. Zheng, Eugene Palovcak, Jean-Paul Armache, Yifan Cheng, David A. Agard
bioRxiv 061960; doi: https://doi.org/10.1101/061960

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