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Measurements of translation initiation from all 64 codons in E. coli

Ariel Hecht, Jeff Glasgow, Paul R. Jaschke, Lukmaan Bawazer, Matthew S. Munson, Jennifer Cochran, Drew Endy, Marc Salit
doi: https://doi.org/10.1101/063800
Ariel Hecht
1Joint Initiative for Metrology in Biology, Stanford, CA
2Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA
3Department of Bioengineering, Stanford, CA
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Jeff Glasgow
1Joint Initiative for Metrology in Biology, Stanford, CA
2Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA
3Department of Bioengineering, Stanford, CA
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Paul R. Jaschke
3Department of Bioengineering, Stanford, CA
4Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia
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Lukmaan Bawazer
1Joint Initiative for Metrology in Biology, Stanford, CA
2Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA
3Department of Bioengineering, Stanford, CA
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Matthew S. Munson
1Joint Initiative for Metrology in Biology, Stanford, CA
2Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA
3Department of Bioengineering, Stanford, CA
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Jennifer Cochran
1Joint Initiative for Metrology in Biology, Stanford, CA
3Department of Bioengineering, Stanford, CA
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Drew Endy
1Joint Initiative for Metrology in Biology, Stanford, CA
3Department of Bioengineering, Stanford, CA
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  • For correspondence: salit@nist.gov. endy@stanford.edu.
Marc Salit
1Joint Initiative for Metrology in Biology, Stanford, CA
2Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA
3Department of Bioengineering, Stanford, CA
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  • For correspondence: salit@nist.gov. endy@stanford.edu.
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ABSTRACT

Our understanding of translation is one cornerstone of molecular biology that underpins our capacity to engineer living matter. The canonical start codon (AUG) and a few near-cognates (GUG, UUG) are typically considered as the “start codons” for translation initiation in Escherichia coli (E. coli). Translation is typically not thought to initiate from the 61 remaining codons. Here, we systematically quantified translation initiation in E. coli from all 64 triplet codons. We detected protein synthesis above background initiating from at least 46 codons. Translation initiated from these non-canonical start codons at levels ranging from 0.01% to 2% relative to AUG. Translation initiation from non-canonical start codons may contribute to the synthesis of peptides in both natural and synthetic biological systems

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 14, 2016.
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Measurements of translation initiation from all 64 codons in E. coli
Ariel Hecht, Jeff Glasgow, Paul R. Jaschke, Lukmaan Bawazer, Matthew S. Munson, Jennifer Cochran, Drew Endy, Marc Salit
bioRxiv 063800; doi: https://doi.org/10.1101/063800
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Measurements of translation initiation from all 64 codons in E. coli
Ariel Hecht, Jeff Glasgow, Paul R. Jaschke, Lukmaan Bawazer, Matthew S. Munson, Jennifer Cochran, Drew Endy, Marc Salit
bioRxiv 063800; doi: https://doi.org/10.1101/063800

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