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Identification of a novel putative interaction partner of the nucleoporin ALADIN

View ORCID ProfileRamona Jühlen, Dana Landgraf, Angela Huebner, Katrin Koehler
doi: https://doi.org/10.1101/064766
Ramona Jühlen
aKlinik und Poliklinik für Kinder- und Jugendmedizin, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Germany
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  • ORCID record for Ramona Jühlen
  • For correspondence: ramona.juehlen@uniklinikum-dresden.de
Dana Landgraf
aKlinik und Poliklinik für Kinder- und Jugendmedizin, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Germany
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Angela Huebner
aKlinik und Poliklinik für Kinder- und Jugendmedizin, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Germany
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Katrin Koehler
aKlinik und Poliklinik für Kinder- und Jugendmedizin, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Germany
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Abstract

It has been shown that the nucleoporin ALADIN employs a significant role in the redox homeostasis of the cell but the function in steroidogenesis contributing to adrenal atrophy in triple A syndrome remains largely unknown. In an attempt to identify new interaction partners of ALADIN, co-immunoprecipitation followed by proteome analysis was conducted in different expression models using the human adrenocortical tumour cell line NCI-H295R. Our results suggest an interaction of ALADIN with the microsomal protein PGRMC2. PGRMC2 is shown to be activity regulator of CYP P450 enzymes and therefore, to be a possible target for adrenal dysregulation in triple A syndrome. We show that there is a sexual dimorphism regarding the expression of Pgrmc2 in adrenals and gonads of WT and Aaas KO mice. Female Aaas KO mice are sterile due to delayed oocyte maturation and meiotic spindle assembly. A participation in meiotic spindle assembly confirms the recently investigated involvement of ALADIN in mitosis and emphasises an interaction with PGRMC2 which is a regulator of cell cycle. By identification of a novel interaction partner of ALADIN we provide novel aspects for future research of the function of ALADIN during cell cycle and for new insights into the pathogenesis of triple A syndrome.

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Posted July 19, 2016.
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Identification of a novel putative interaction partner of the nucleoporin ALADIN
Ramona Jühlen, Dana Landgraf, Angela Huebner, Katrin Koehler
bioRxiv 064766; doi: https://doi.org/10.1101/064766
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Identification of a novel putative interaction partner of the nucleoporin ALADIN
Ramona Jühlen, Dana Landgraf, Angela Huebner, Katrin Koehler
bioRxiv 064766; doi: https://doi.org/10.1101/064766

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