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Insights into grapevine defense response against drought as revealed by biochemical, physiological and RNA-Seq analysis

Muhammad Salman Haider, Cheng Zhang, Mahantesh M. Kurjogi Tariq Pervaiz, Ting Zheng, Chao bo Zhang, Chen Lide, Lingfie Shangguan, Jinggui Fang
doi: https://doi.org/10.1101/065136
Muhammad Salman Haider
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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Cheng Zhang
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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Mahantesh M. Kurjogi Tariq Pervaiz
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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Ting Zheng
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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Chao bo Zhang
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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Chen Lide
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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Lingfie Shangguan
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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Jinggui Fang
College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China
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  • For correspondence: fanggg@njau.edu.cn
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Abstract

Grapevine is economically important and widely cultivated fruit crop, which is seriously hampered by drought worldwide. It is necessary to understand the impact of glitches incurred by the drought on grapevine genetic resources. Therefore, in the present study RNA-sequencing analysis was performed using cDNA libraries constructed from both drought-stress and control plants. Results yielded, a total of 12,451 differentially expressed genes (DEGs) out of which 8,022 genes were up-regulated and 4,430 were down-regulated. Further physiological and biochemical analyses were carried out to validate the various biological processes involved in the development of grapevine in response to drought stress. Results also showed that decrease in rate of stomatal conductance in-turn decrease the photosynthetic activity and CO2 assimilation rate in the grapevine leaves and most ROS detoxification systems, including stress enzymes, stress related proteins and secondary metabolites were strongly induced. Moreover, various hormones were known to be induced in the present study in response to drought. Overall the present study concludes that these DEGs play both positive and negative role in drought tolerance by regulating different biological pathways of grapevine. However our findings have provided valuable gene information for future studies of abiotic stress in grapevine and other fruit crops.

  • Abbreviations

    ABA
    Abscisic acid
    ANOVA
    Analysis of variance
    BR
    Brassinosteroid
    CAT
    Catalase
    DEGs
    Differentially-expressed genes
    ESTs
    Expressed sequence tags
    FDR
    False discovery rate
    GA
    Gibberellic acid
    IAA
    Indole-acetic acid
    JA
    Jasmonic acid
    MARS
    MA-plot-based method with Random Sampling model
    MDA
    Malondialdehyde
    POD
    peroxidase
    qRT-PCR
    quantitative real-time PCR
    RNA-seq
    RNA-sequencing
    ROS
    Reactive oxygen species
    SD
    Standard deviation
    SOD
    Superoxide dismutase
  • Copyright 
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    Posted December 19, 2016.
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    Insights into grapevine defense response against drought as revealed by biochemical, physiological and RNA-Seq analysis
    Muhammad Salman Haider, Cheng Zhang, Mahantesh M. Kurjogi Tariq Pervaiz, Ting Zheng, Chao bo Zhang, Chen Lide, Lingfie Shangguan, Jinggui Fang
    bioRxiv 065136; doi: https://doi.org/10.1101/065136
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    Insights into grapevine defense response against drought as revealed by biochemical, physiological and RNA-Seq analysis
    Muhammad Salman Haider, Cheng Zhang, Mahantesh M. Kurjogi Tariq Pervaiz, Ting Zheng, Chao bo Zhang, Chen Lide, Lingfie Shangguan, Jinggui Fang
    bioRxiv 065136; doi: https://doi.org/10.1101/065136

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