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Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina

View ORCID ProfileJaroslav Icha, View ORCID ProfileChristiane Grunert, Mauricio Rocha-Martins, Caren Norden
doi: https://doi.org/10.1101/066761
Jaroslav Icha
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
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  • ORCID record for Jaroslav Icha
Christiane Grunert
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
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Mauricio Rocha-Martins
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
2Instituto de Biofísica Carlos Chagas Filho, Av. Carlos Chagas Filho 373, 21941-902 Rio de Janeiro, Brazil
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Caren Norden
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
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  • For correspondence: norden@mpi-cbg.de
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Abstract

The arrangement of neurons into distinct layers is critical for neuronal connectivity and function of the nervous system. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. Here, we investigate ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited, RGCs that fail to translocate induce lamination defects, indicating that correct RGC translocation is crucial for subsequent retinal lamination.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 29, 2016.
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Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina
Jaroslav Icha, Christiane Grunert, Mauricio Rocha-Martins, Caren Norden
bioRxiv 066761; doi: https://doi.org/10.1101/066761
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Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina
Jaroslav Icha, Christiane Grunert, Mauricio Rocha-Martins, Caren Norden
bioRxiv 066761; doi: https://doi.org/10.1101/066761

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