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Highly parallel direct RNA sequencing on an array of nanopores

Daniel R Garalde, Elizabeth A Snell, Daniel Jachimowicz, Andrew J Heron, Mark Bruce, Joseph Lloyd, Anthony Warland, Nadia Pantic, Tigist Admassu, Jonah Ciccone, Sabrina Serra, Jemma Keenan, Samuel Martin, Luke McNeill, Jayne Wallace, Lakmal Jayasinghe, Chris Wright, Javier Blasco, Botond Sipos, Stephen Young, Sissel Juul, James Clarke, Daniel J Turner
doi: https://doi.org/10.1101/068809
Daniel R Garalde
Oxford Nanopore Technologies
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Elizabeth A Snell
Oxford Nanopore Technologies
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Daniel Jachimowicz
Oxford Nanopore Technologies
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Andrew J Heron
Oxford Nanopore Technologies
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Mark Bruce
Oxford Nanopore Technologies
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Joseph Lloyd
Oxford Nanopore Technologies
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Anthony Warland
Oxford Nanopore Technologies
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Nadia Pantic
Oxford Nanopore Technologies
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Tigist Admassu
Oxford Nanopore Technologies
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Jonah Ciccone
Oxford Nanopore Technologies
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Sabrina Serra
Oxford Nanopore Technologies
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Jemma Keenan
Oxford Nanopore Technologies
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Samuel Martin
Oxford Nanopore Technologies
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Luke McNeill
Oxford Nanopore Technologies
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Jayne Wallace
Oxford Nanopore Technologies
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Lakmal Jayasinghe
Oxford Nanopore Technologies
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Chris Wright
Oxford Nanopore Technologies
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Javier Blasco
Oxford Nanopore Technologies
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Botond Sipos
Oxford Nanopore Technologies
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Stephen Young
Oxford Nanopore Technologies
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Sissel Juul
Oxford Nanopore Technologies
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James Clarke
Oxford Nanopore Technologies
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Daniel J Turner
Oxford Nanopore Technologies
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  • For correspondence: dan.turner@nanoporetech.com
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Abstract

Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing -- information that is useful for understanding the status and function of a sample. Nanopore-based sequencing technology is capable of electronically analysing a sample's DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology which can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, strand-specific RNA sequences. This will improve the ease and speed of RNA analysis, while yielding richer biological information.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 12, 2016.
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Highly parallel direct RNA sequencing on an array of nanopores
Daniel R Garalde, Elizabeth A Snell, Daniel Jachimowicz, Andrew J Heron, Mark Bruce, Joseph Lloyd, Anthony Warland, Nadia Pantic, Tigist Admassu, Jonah Ciccone, Sabrina Serra, Jemma Keenan, Samuel Martin, Luke McNeill, Jayne Wallace, Lakmal Jayasinghe, Chris Wright, Javier Blasco, Botond Sipos, Stephen Young, Sissel Juul, James Clarke, Daniel J Turner
bioRxiv 068809; doi: https://doi.org/10.1101/068809
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Highly parallel direct RNA sequencing on an array of nanopores
Daniel R Garalde, Elizabeth A Snell, Daniel Jachimowicz, Andrew J Heron, Mark Bruce, Joseph Lloyd, Anthony Warland, Nadia Pantic, Tigist Admassu, Jonah Ciccone, Sabrina Serra, Jemma Keenan, Samuel Martin, Luke McNeill, Jayne Wallace, Lakmal Jayasinghe, Chris Wright, Javier Blasco, Botond Sipos, Stephen Young, Sissel Juul, James Clarke, Daniel J Turner
bioRxiv 068809; doi: https://doi.org/10.1101/068809

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