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Natural and laboratory mutations in kuzbanian are associated with heavy metal stress phenotypes in Drosophila melanogaster

Hung Le Manh, Lain Guio, Miriam Merenciano, Quirze Rovira, Maite G. Barrón, Josefa González
doi: https://doi.org/10.1101/070243
Hung Le Manh
1Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49. 08003 Barcelona. Spain
2Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st, Hanoi, Vietnam
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Lain Guio
1Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49. 08003 Barcelona. Spain
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Miriam Merenciano
1Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49. 08003 Barcelona. Spain
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Quirze Rovira
1Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49. 08003 Barcelona. Spain
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Maite G. Barrón
1Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49. 08003 Barcelona. Spain
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Josefa González
1Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49. 08003 Barcelona. Spain
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  • For correspondence: josefa.gonzalez@ibe.upf-csic.es
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ABSTRACT

Organisms must cope with altered environmental conditions such as high concentrations of heavy metals. Stress response to heavy metals is mediated by the metal-responsive transcription factor 1 (MTF-1), which is conserved from Drosophila to humans. MTF-1 binds to metal response elements (MREs) and changes the expression of target genes. kuzbanian (kuz), a metalloendopeptidase that activates the evolutionary conserved Notch signaling pathway, has been identified as an MTF-1 target gene. We have previously identified a putatively adaptive transposable element in the Drosophila melanogaster genome, named FBti0019170, inserted in a kuz intron. In this work, we investigated whether laboratory-induced mutations in kuz are associated with zinc stress phenotypes. We found that both embryos and adult flies overexpressing kuz are more tolerant to zinc compared with wild-type flies. On the other hand, we found that the effect of FBti0019170 on zinc stress tolerance depends on developmental stage and genetic background. Moreover, in the majority of the genetic backgrounds analyzed, FBti0019170 has a deleterious effect in unpolluted environments in pre-adult stages. These results highlight the complexity of natural mutations and suggest that besides laboratory-induced mutations natural mutations need to be studied in order to accurately characterize gene function and evolution.

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Posted August 18, 2016.
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Natural and laboratory mutations in kuzbanian are associated with heavy metal stress phenotypes in Drosophila melanogaster
Hung Le Manh, Lain Guio, Miriam Merenciano, Quirze Rovira, Maite G. Barrón, Josefa González
bioRxiv 070243; doi: https://doi.org/10.1101/070243
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Natural and laboratory mutations in kuzbanian are associated with heavy metal stress phenotypes in Drosophila melanogaster
Hung Le Manh, Lain Guio, Miriam Merenciano, Quirze Rovira, Maite G. Barrón, Josefa González
bioRxiv 070243; doi: https://doi.org/10.1101/070243

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