Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Strigolactone regulates shoot development through a core signalling pathway

Tom Bennett, Yueyang Liang, Madeleine Seale, Sally Ward, Dörte Müller, View ORCID ProfileOttoline Leyser
doi: https://doi.org/10.1101/070763
Tom Bennett
1Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK
2Current Address: Department of Biology, University of Leeds, Leeds, LS2 9JT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yueyang Liang
1Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Madeleine Seale
1Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK
3Current Address: Institute of Molecular Plant Sciences, University of Edinburgh, Edinburgh, EH9 3BF, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sally Ward
1Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dörte Müller
4Department of Biology, University of York, York, YO10 5DD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ottoline Leyser
1Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK
4Department of Biology, University of York, York, YO10 5DD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ottoline Leyser
  • For correspondence: ol235@cam.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Strigolactones are a recently identified class of hormone that regulate multiple aspects of plant development. The DWARF14 (D14) α/β fold protein has been identified as a strigolactone receptor, which can act through the SCFMAX2 ubiquitin ligase, but the universality of this mechanism is not clear. Multiple proteins have been suggested as targets for strigolactone signalling, including both direct proteolytic targets of SCFMAX2, and downstream targets. However, the relevance and importance of these proteins to strigolactone signalling in many cases has not been fully established. Here we assess the contribution of these targets to strigolactone signalling in adult shoot developmental responses. We find that all examined strigolactone responses are regulated by SCFMAX2 and D14, and not by other D14-like proteins. We further show that all examined strigolactone responses likely depend on degradation of SMXL proteins in the SMXL6 clade, and not on other proposed proteolytic targets. Taken together, our results suggest that in the adult shoot, the dominant mode of strigolactone signalling is D14-initiated, MAX2-mediated degradation of SMXL6-related proteins. We confirm that the BRANCHED1 transcription factor and the PIN-FORMED1 auxin efflux carrier are plausible downstream targets of this pathway in the regulation of shoot branching, and show that BRC1 likely acts in parallel to PIN1.

AUTHOR SUMMARY Strigolactones are a recently discovered family plant hormones with diverse roles in development, most strikingly in the regulation shoot branching. Our understanding of the mechanism(s) by which plants perceive and respond to strigolactones is growing rapidly. It is likely that the strigolactone signaling pathway has evolved by duplication and diversification of specific components of a pre-existing pathway, involved in perception and response to an as yet unknown hormone. Several of these components have been identified and several new candidate components have been implicated in the pathway. We have adopted a genetic approach to assess systematically the contributions of all these players to strigolactone signaling in the shoot. We exclude some of the candidate proteins from involvement in strigolactone-mediated shoot branching control and define a core pathway for strigolactone action in the shoot. We provide evidence that downstream of this core, the strigolactone signaling pathway branches, with different effectors mediating different shoot responses.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted August 21, 2016.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Strigolactone regulates shoot development through a core signalling pathway
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Strigolactone regulates shoot development through a core signalling pathway
Tom Bennett, Yueyang Liang, Madeleine Seale, Sally Ward, Dörte Müller, Ottoline Leyser
bioRxiv 070763; doi: https://doi.org/10.1101/070763
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Strigolactone regulates shoot development through a core signalling pathway
Tom Bennett, Yueyang Liang, Madeleine Seale, Sally Ward, Dörte Müller, Ottoline Leyser
bioRxiv 070763; doi: https://doi.org/10.1101/070763

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4397)
  • Biochemistry (9629)
  • Bioengineering (7123)
  • Bioinformatics (24937)
  • Biophysics (12670)
  • Cancer Biology (9994)
  • Cell Biology (14400)
  • Clinical Trials (138)
  • Developmental Biology (7989)
  • Ecology (12147)
  • Epidemiology (2067)
  • Evolutionary Biology (16025)
  • Genetics (10951)
  • Genomics (14778)
  • Immunology (9905)
  • Microbiology (23739)
  • Molecular Biology (9506)
  • Neuroscience (51049)
  • Paleontology (370)
  • Pathology (1545)
  • Pharmacology and Toxicology (2692)
  • Physiology (4038)
  • Plant Biology (8693)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2404)
  • Systems Biology (6458)
  • Zoology (1350)