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Bovine Lactoferrin Activity Against Chikungunya and Zika Viruses

Carlos A. M. Carvalho, Samir M. M. Casseb, Rafael B. Gonçalves, Eliana V. P. Silva, Andre M. O. Gomes, Pedro F. C. Vasconcelos
doi: https://doi.org/10.1101/071571
Carlos A. M. Carvalho
1Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, Ananindeua, Pará, Brazil
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  • For correspondence: c_marques@live.com
Samir M. M. Casseb
1Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, Ananindeua, Pará, Brazil
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Rafael B. Gonçalves
2Departamento de Bioquímica, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
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Eliana V. P. Silva
1Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, Ananindeua, Pará, Brazil
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Andre M. O. Gomes
3Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
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Pedro F. C. Vasconcelos
1Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, Ananindeua, Pará, Brazil
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Abstract

Chikungunya (CHIKV) and Zika (ZIKV) viruses are two arboviruses which have recently broken their sylvatic isolation and gone into a rampant spreading among humans in some urban areas of the world, specially in Latin America. Given the huge burden that Chikungunya and Zika fevers impose to public health in the affected countries and the lack of effective interventions against them, the aim of this work was to evaluate the antiviral potential of bovine lactoferrin (bLf) – an iron-binding glycoprotein with broad-spectrum antimicrobial properties – in both CHIKV and ZIKV infections. The general antiviral activity of bLf was assessed by plaque assays, and the inhibitory effects of the protein on specific stages of virus infecion was evaluated by immunofluorescence and nucleic acid quantification assays. Our data show that bLf exerts a dose-dependent strong inhibitory effect on the infection of Vero cells by the aforementioned arboviruses, reducing their infection efficiency in up to nearly 80%, with no significant cytotoxicity, and such antiviral activity occurs at the levels of binding and replication of the virus particles. Taken together, these findings reveal that bLf antimicrobial properties are extendable to CHIKV and ZIKV, underlining a generic inhibition mechanism that can be explored to develop a potential strategy against their infections.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 25, 2016.
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Bovine Lactoferrin Activity Against Chikungunya and Zika Viruses
Carlos A. M. Carvalho, Samir M. M. Casseb, Rafael B. Gonçalves, Eliana V. P. Silva, Andre M. O. Gomes, Pedro F. C. Vasconcelos
bioRxiv 071571; doi: https://doi.org/10.1101/071571
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Bovine Lactoferrin Activity Against Chikungunya and Zika Viruses
Carlos A. M. Carvalho, Samir M. M. Casseb, Rafael B. Gonçalves, Eliana V. P. Silva, Andre M. O. Gomes, Pedro F. C. Vasconcelos
bioRxiv 071571; doi: https://doi.org/10.1101/071571

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