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MAPPING OF LONG-RANGE CHROMATIN INTERACTIONS BY PROXIMITY LIGATION ASSISTED CHIP-SEQ

Rongxin Fang, Miao Yu, Guoqiang Li, Sora Chee, Tristin Liu, Bing Ren
doi: https://doi.org/10.1101/074294
Rongxin Fang
1Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA.
2Bioinformatics and Systems Biology Graduate Program, University of California, San Diego, La Jolla, California 92093, USA.
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Miao Yu
1Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA.
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Guoqiang Li
1Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA.
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Sora Chee
1Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA.
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Tristin Liu
1Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA.
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Bing Ren
1Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA.
3Department of Cellular and Molecular Medicine, Institute of Genomic Medicine, and Moores Cancer Center, University of California at San Diego, La Jolla, CA 92093, USA.
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  • For correspondence: biren@ucsd.edu
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Abstract

We report a highly sensitive and cost-effective method for genome-wide identification of chromatin interactions in eukaryotic cells. Combining proximity ligation with chromatin immunoprecipitation and sequencing, the method outperforms the state of art approach in sensitivity, accuracy and ease of operation. Application of the method to mouse embryonic stem cells improves mapping of enhancer-promoter interactions.

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Posted September 09, 2016.
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MAPPING OF LONG-RANGE CHROMATIN INTERACTIONS BY PROXIMITY LIGATION ASSISTED CHIP-SEQ
Rongxin Fang, Miao Yu, Guoqiang Li, Sora Chee, Tristin Liu, Bing Ren
bioRxiv 074294; doi: https://doi.org/10.1101/074294
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MAPPING OF LONG-RANGE CHROMATIN INTERACTIONS BY PROXIMITY LIGATION ASSISTED CHIP-SEQ
Rongxin Fang, Miao Yu, Guoqiang Li, Sora Chee, Tristin Liu, Bing Ren
bioRxiv 074294; doi: https://doi.org/10.1101/074294

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