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Genome‑wide identification and characterization of Transcription Factors of Basic Leucine Zipper Family in Malus domestica

Zhengrong Zhang, Haoran Cui, Shanshan Xing, Xin Liu, Xuesen Chen, Xiaoyun Wang
doi: https://doi.org/10.1101/075994
Zhengrong Zhang
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Shandong Taian 271018, People’s Republic of china
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Haoran Cui
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Shandong Taian 271018, People’s Republic of china
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Shanshan Xing
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Shandong Taian 271018, People’s Republic of china
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Xin Liu
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Shandong Taian 271018, People’s Republic of china
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Xuesen Chen
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Shandong Taian 271018, People’s Republic of china
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Xiaoyun Wang
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Shandong Taian 271018, People’s Republic of china
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  • For correspondence: xyunwang@sdau.edu.cn
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Abstract

Basic leucine zipper proteins (bZIP) contain a basic DNA-binding region and a leucine zipper region, acting as transcriptional factors in regulation of gene expression exclusively in eukaryotes. In this investigation, total 116 bZIP members were identified in apple genome and mapped on all 17 chromosomes with various densities as M.bZIPs. All these members were divided into six groups according to the phylogenetic relationship combining with bZIPs from rice and Arabidopsis. Investigating gene structure of M.bZIPs, five splicing patterns of intron were found in the DNA-binging region with no splicing position and splicing positions at different nucleotide of codons or different positions. Analyzing of protein structure of M.bZIPs, twenty-five motifs were identified with certain characteristic in different phylogenetic groups. To predict dimerization of leucine zipper region, the key positions of amino acid in heptad(s) were investigated. The results showed that most M.bZIPs may form hetero-dimer or homo-dimer and some M.bZIPs may form both. Expression experiment revealed that M.bZIP genes have organ-specific expression and widely expressed in flowers, leaves, and fruits. To investigate the response of M.bZIPs to abiotic stresses, the promoter sequences of randomly selected M.bZIP genes were analyzed. Cis-acting elements related to multiple stresses were found existing widely in promoter sequences. Quantitative real-time PCR results further demonstrated that the expression of some M.bZIP genes were quite sensitive to exogenous abscisic acid and osmotic treatments.

  • Abbreviations

    ABA
    abscisic acid
    ABRE
    abscisic acid responsive elements
    bZIP gene
    basic leucine zipper encoding gene
    DRE
    dehydration-response element
    LTRE
    low-temperature responsive element
    qRT-PCR
    quantitative real-time PCR
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    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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    Posted September 19, 2016.
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    Genome‑wide identification and characterization of Transcription Factors of Basic Leucine Zipper Family in Malus domestica
    Zhengrong Zhang, Haoran Cui, Shanshan Xing, Xin Liu, Xuesen Chen, Xiaoyun Wang
    bioRxiv 075994; doi: https://doi.org/10.1101/075994
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    Genome‑wide identification and characterization of Transcription Factors of Basic Leucine Zipper Family in Malus domestica
    Zhengrong Zhang, Haoran Cui, Shanshan Xing, Xin Liu, Xuesen Chen, Xiaoyun Wang
    bioRxiv 075994; doi: https://doi.org/10.1101/075994

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